| 摘要: |
| 目的 观察结膜黏膜相关淋巴组织（MALT）淋巴瘤中microRNA-150（miR-150）的表达，探讨miR-150在结膜MALT淋巴瘤中影响肿瘤细胞增殖、迁移与侵袭的机制。方法 使用qPCR法检测第二军医大学长征医院收治的3例结膜MALT淋巴瘤患者肿瘤组织及瘤旁组织中miR-150及其可能的下游靶分子Cbl-b的表达。将miR-150抑制物和阴性对照转染人多发性骨髓瘤细胞株RPMI 8226，采用CCK-8法和流式细胞术研究miR-150对RPMI 8226细胞增殖和凋亡的影响，通过Transwell实验研究miR-150对RPMI 8226细胞迁移和侵袭的影响，用蛋白质印迹法检测miR-150对RPMI 8226细胞中Cbl-b表达的调控。结果 与瘤旁组织相比，结膜MALT淋巴瘤组织中miR-150表达上调（P<0.05，P<0.01）；抑制miR-150后，RPMI 8226细胞的增殖受到抑制，细胞凋亡明显增加，迁移和侵袭能力降低，与阴性对照组相比差异有统计学意义（P<0.05，P<0.01）。在结膜MALT淋巴瘤组织中，miR-150下游靶基因Cbl-b表达下调（P<0.01）；抑制miR-150后，RPMI 8226细胞内Cbl-b蛋白的表达上调（P<0.01）。结论 MiR-150对淋巴瘤细胞的增殖、迁移和侵袭有促进作用，其表达上调参与了MALT淋巴瘤的发生。其机制可能与miR-150对下游分子Cbl-b的抑制性调控有关。 |
| 关键词: 结膜肿瘤 黏膜相关淋巴组织淋巴瘤 microRNA-150 Cbl-b 细胞增殖 细胞迁移 肿瘤侵袭 |
| DOI：10.16781/j.0258-879x.2017.06.0727 |
| 投稿时间：2017-02-24修订日期：2017-05-31 |
| 基金项目:上海市自然科学基金（14ZR1414000），上海市卫生和计划生育委员会科研课题面上项目（201445） |
|
| Role of micro RNA-150 in proliferation, migration and invasion of conjunctival mucosa-associated lymphoid tissue lymphoma |
| LI Yu-zhen,QIAN Yu,SHAN Yu,LIANG Wen-jun,WEI Rui-li* |
(Department of Ophthalmology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
*Corresponding author) |
| Abstract: |
| Objective To observe the expression of microRNA-150 (miR-150) in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma, so as to investigate its mechanism in proliferation, migration and invasion of conjunctival MALT lymphoma. Methods The expressions of miR-150 and Cbl-b, a possible downstream molecule of miR-150, were measured by qPCR in MALT lymphoma tissues and precancerous tissues collected from 3 patients with conjunctival MALT lymphoma in Changzheng Hospital of Second Military Medical University. Then, we transfected miR-150 inhibitor and negative control into human multiple myeloma cell lines RPMI 8226 by cell transfection. CCK-8 assay and flow cytometry (FCM) method were used to investigate the role of miR-150 in the proliferation and apoptosis of RPMI 8226 cells. Transwell assay was used to analyze the effect of miR-150 on the migration and invasion of RPMI 8226 cells. Western blotting analysis was used to examine the regulation of miR150 on Cbl-b expression in RPMI 8226 cells. Results The expression of miR-150 was significantly increased in the conjunctival MALT lymphoma tissues compared with precancerous tissues (P<0.05,P<0.01). Compared with negative control group, the proliferation of RPMI 8226 cells was significantly repressed (P<0.01), the apoptosis was significantly increased (P<0.01), and the migration and invasion were significantly decreased (P<0.05,P<0.01) after transfection of miR-150 inhibitor. The expression of Cbl-b was significantly up-regulated in MALT lymphoma tissues, and was significantly increased after inhibiting miR-150 expression. Conclusion Up-regulated miR-150 can promote the proliferation, migration and invasion of lymphoma cells and is involved in the generation of conjunctival MALT lymphoma, which may be mediated by inhibiting its downstream target gene Cbl-b. |
| Key words: conjunctival neoplasms mucosa-associated lymphoid tissue lymphoma microRNA-150 Cbl-b cell proliferation cell migration neoplasm invasiveness |
