| 摘要: |
| 目的 检测慢性脑低灌注性血管性认知损害（CCH-VCI）患者血清p75神经营养素受体细胞外段（p75NTR-ECD）水平，并探讨其与肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、IL-6的关系。方法 选择海军军医大学（第二军医大学）长海医院2018年8月至12月收治的34例CCH-VCI患者，以及同期相同年龄段的36名中老年健康人和34例缺血性脑卒中患者作为研究对象。采用酶联免疫吸附试验测定3组研究对象的血清p75NTR-ECD、TNF-α、IL-1β、IL-6水平并进行组间比较。采用Spearman相关分析研究CCH-VCI患者血清p75NTR-ECD水平与TNF-α、IL-1β、IL-6水平的相关性。结果 CCH-VCI组血清p75NTR-ECD水平高于健康对照组和缺血性脑卒中组[（544.36（440.88，628.50）pg/mL vs 276.49（262.59，313.87）pg/mL、366.87（337.09，450.43）pg/mL]，差异均有统计学意义（U=87.500、335.500，P均<0.05）。CCH-VCI组患者血清TNF-α、IL-1β、IL-6水平分别为196.02（141.20，280.35）pg/mL、68.23（60.79，91.94）pg/mL、51.04（40.24，65.26）pg/mL，缺血性脑卒中组分别为218.67（143.76，281.28）pg/mL、76.87（59.10，99.91）pg/mL、64.45（43.13，86.76）pg/mL，均分别高于健康对照组[分别为73.71（56.94，79.81）pg/mL、42.98（34.52，51.34）pg/mL、14.97（11.76，21.19）pg/mL]，差异均有统计学意义（U=31.000、4.000，106.000、132.000，48.000、13.000；P均<0.05）。CCH-VCI患者血清p75NTR-ECD水平与TNF-α水平存在相关性（r=0.391，P=0.022），但与IL-1β和IL-6水平均无明显相关性（r=0.032、0.164，P=0.855、0.355）。结论 慢性脑低灌注损伤后p75NTR可能与TNF-α等炎性因子有关，并共同参与了CCH-VCI的发病。 |
| 关键词: 脑低灌注 血管性认知损害 p75神经营养素受体细胞外段 炎性因子 |
| DOI：10.16781/j.0258-879x.2019.06.0602 |
| 投稿时间：2019-01-14修订日期：2019-03-21 |
| 基金项目:上海市科委医学引导类项目（124119a8900）. |
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| Serum p75 neurotrophin receptor-extracellular domain level and its relationship with inflammatory factors in patients with chronic cerebral hypoperfusion-vascular cognitive impairment |
| CHEN Jia,QIN Lu-ping,LUO Meng,ZHAO Ming,CHEN Lei,WU Tao,DENG Ben-qiang* |
(Stroke Center, Changhai Hospital, Naval Medical University(Second Military Medical University), Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Objective To investigate serum p75 neurotrophin receptor-extracellular domain (p75NTR-ECD) level in patients with chronic cerebral hypoperfusion-vascular cognitive impairment (CCH-VCI) and its relationship with tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-6. Methods The clinical data of patients with CCH-VCI (n=34) were collected from Changhai Hospital, Naval Medical University (Second Military Medical University) from Aug. to Dec. 2018. Enzyme linked immunosorbent assay was applied for detection of serum levels of p75NTR-ECD, TNF-α, IL-1β and IL-6; and the results were then compared with those of ischemic stroke participants (n=34) and healthy controls (n=36), who were all in the same age range. Spearman correlation analysis was used to analyze the relationship between serum p75NTR-ECD level and the above-mentioned inflammatory factors in CCH-VCI patients. Results The serum p75NTR-ECD level in the CCH-VCI group was significantly higher than those in the healthy control group and the ischemic stroke group (544.36[440.88, 628.50] pg/mL vs 276.49[262.59, 313.87] pg/mL and 366.87[337.09, 450.43] pg/mL, U=87.500 and 335.500, both P<0.05). The serum levels of TNF-α, IL-1β and IL-6 were 196.02 (141.20, 280.35) pg/mL, 68.23 (60.79, 91.94) pg/mL and 51.04 (40.24, 65.26) pg/mL in the CCH-VCI group, respectively, and 218.67 (143.76, 281.28) pg/mL, 76.87 (59.10, 99.91) pg/mL and 64.45 (43.13, 86.76) pg/mL in the ischemic stroke group, respectively, which were all significantly higher than those in the healthy control group (73.71[56.94, 79.81] pg/mL, 42.98[34.52, 51.34] pg/mL and 14.97[11.76, 21.19] pg/mL, respectively; U=31.000 and 4.000, 106.000 and 132.000, and 48.000 and 13.000; all P<0.05). Serum p75NTR-ECD level in the CCH-VCI patients was correlated with TNF-α level (r=0.391, P=0.022), but not with IL-1β or IL-6 levels (r=0.032 and 0.164, P=0.855 and 0.355). Conclusion Serum p75NTR level may be related to inflammatory factors (TNF-α) after chronic cerebral hypoperfusion, and they may jointly participate in the pathogenesis of CCH-VCI. |
| Key words: cerebral hypoperfusion vascular cognitive impairment p75 neurotrophin receptor-extracellular domain inflammatory factor |
