| 摘要: |
| 目的 探讨代谢解毒酶活性变化和击倒抗性（kdr）基因突变在白纹伊蚊菊酯类抗性机制中的作用。方法 2017年8月至9月分别在山东省济南市千佛山公园（JN）、浙江省杭州市上茅家埠（HZ）、上海市宝山区宝山六村（BS）、上海市杨浦区共青森林公园（YP）和海南省海口市美兰区居民区（HK）采集现场白纹伊蚊（生物测定均为抗性种群），检测其代谢解毒酶谷胱甘肽S-转移酶（GST）和多功能氧化酶（MFO）的活性并与敏感品系的白纹伊蚊比较，采用分类回归树方法（CART）分析GST和MFO活性变化及kdr突变（I1532和F1534）在抗性产生中的贡献率。结果 白纹伊蚊敏感品系的GST和MFO的活性基线水平均高于现场抗性种群BS和HK（P均<0.01）。BS种群接触溴氰菊酯后与未接触杀虫剂的基线相比，GST和MFO变化不明显（P>0.05），接触氯菊酯后GST活性升高（P<0.05），MFO活性也升高（P<0.01）；HK种群接触溴氰菊酯后GST活性差异不明显（P>0.05）、MFO活性升高（P<0.01），接触氯菊酯后GST和MFO活性变化均不明显（P均>0.05）。5个现场抗性种群接触溴氰菊酯和氯菊酯后的GST和MFO活性与敏感品系的基线相比，变化无规律。CART分析结果显示，白纹伊蚊对溴氰菊酯的抗性产生中GST活性和kdr F1534突变的贡献率较大，其次是MFO活性，kdr I1532突变贡献率最小；对氯菊酯的抗性产生中，kdr F1534突变的贡献率最大，其次是GST和MFO活性，kdr I1532突变无贡献。结论 代谢解毒酶GST和MFO活性水平不适合作为判断白纹伊蚊种群对菊酯类杀虫剂抗性的单因素指标；代谢解毒酶活性变化和kdr突变可能是白纹伊蚊对菊酯类杀虫剂抗性产生中相互协同的2种机制。 |
| 关键词: 白纹伊蚊 除虫菊酯类 代谢抗性 击倒抗性 |
| DOI：10.16781/j.0258-879x.2019.05.0512 |
| 投稿时间：2019-01-30修订日期：2019-03-15 |
| 基金项目: |
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| Role of metabolic detoxifcation enzyme activity and knockdown resistance gene mutations in resistance of Aedes albopictus to pyrethroid insecticides |
| CHEN Han-ming1,GAO Jing-peng1,SHI Hua2,LI Xiang-yu3,PENG Heng3*,MA Ya-jun1* |
(1. Department of Tropical Diseases, Faculty of Naval Medicine, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
2. Center for Disease Control and Prevention of PLA, Beijing 100071, China;
3. Department of Pathogen Biology, College of Basic Medical Sciences, Naval Medical University(Second Military Medical University), Shanghai 200433, China
*Corresponding authors) |
| Abstract: |
| Objective To explore the role of metabolic detoxification enzyme activity and knockdown resistance (kdr) gene mutations in the pyrethroid resistance of Aedes (Ae.) albopictus. Methods From Aug. to Sep. in 2017, the Ae. albopictus samples were collected in Qianfoshan Park, Jinan City, Shandong Province (JN), Shangmaojiabu, Hangzhou City, Zhejiang Province (HZ), Baoshan Sixth Village, Baoshan District, Shanghai (BS), Gongqing Forest Park, Yangpu District, Shanghai (YP), and Meilan District Residential Area, Haikou City, Hainan Province (HK). The above five field populations were all resistant to insecticide. The activities of metabolic detoxification enzymes (glutathione-S transferase[GST] and mixed function oxidase[MFO]) were detected and compared with the Ae. albopictus susceptible strain (JS). The contribution rates of activity changes of GST and MFO and kdr mutations (I1532 and F1534) in the resistance formation were analyzed by the classification and regression trees (CART). Results The baseline enzyme activities of GST and MFO in Ae. albopictus JS were both significantly higher than those in the BS and HK resistant populations (both P<0.01). There were no significant difference in the activities of GST and MFO between the BS population unexposed and exposed to deltamethrin (P>0.05). After exposure to permethrin of BS population, the activities of GST and MFO were significantly increased (P<0.05, P<0.01). After exposure to deltamethrin, the GST activity was not significantly changed in the HK population (P>0.05), while the MFO activity was significantly increased (P<0.01). However, after exposure to permethrin in the HK population, there were no significant changes in the GST and MFO activities (both P>0.05). In the 5 field resistant populations exposed to deltamethrin and permethrin, the changes of GST and MFO activities were irregular compared with baseline of Ae. albopictus JS strain. CART analysis showed that in the resistance formation of Ae. albopictus against deltamethrin, the contribution rates of GST activity and kdr F1534 mutation were the greatest, followed by MFO activity, and the kdr I1532 mutation was the smallest. In the resistance formation of Ae. albopictus against permethrin, the kdr F1534 mutation had the highest contribution rate, followed by the GST and MFO activities, and the kdr I1532 mutation had no contribution. Conclusion The activity levels of metabolic detoxification enzymes (GST and MFO) are not suitable as single markers for detecting the resistance of Ae. albopictus to pyrethroids. The activity changes of metabolic detoxification enzymes and kdr mutations may be two synergistic mechanisms in the resistance formation of Ae. albopictus to pyrethroid insecticides. |
| Key words: Aedes albopictus pyrethroids metabolic resistance knockdown resistance |
