| 摘要: |
| 目的 利用近红外光谱法建立快速测定甘草饮片中甘草酸和甘草苷含量的模型。方法 采用HPLC测定不同产地的甘草饮片中甘草酸和甘草苷的含量作为参考值，分别选取2200~2049、1750~1450、1151~1001nm和1795~1475、1395~1293、1125~1030nm波长范围的近红外光谱，利用偏最小二乘（PLS）回归分析结合交叉验证法，建立快速测定甘草饮片中甘草酸和甘草苷含量的模型。结果 所建立的甘草酸和甘草苷含量校正模型的相关系数分别为0.980和0.919，交互验证均方差分别为0.184和0.144。结论 近红外光谱法结合PLS法操作简便、快速、无损，适合大批甘草饮片中甘草酸和甘草苷的含量测定，为快速评价甘草饮片质量提供了一种新方法。 |
| 关键词: 近红外光谱法 甘草 饮片 甘草酸 甘草苷 快速测定 |
| DOI：10.16781/j.0258-879x.2020.08.0921 |
| 投稿时间：2019-10-08修订日期：2019-12-13 |
| 基金项目:军队医疗机构制剂标准提高计划科研专项课题（13ZJZ11-3，14ZJZ02-3）． |
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| Near-infrared spectroscopy in rapid determination of glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian |
| GAO Hong-bin1,LIU Hao2,XIA Hou-hao3,SONG Hong-jie1* |
(1. Department of Pharmacy, Changhai Hospital, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
2. Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Food and Drug Vocational College, Guangzhou 510520, Guangdong, China;
3. Department of Pharmacy, Bengbu Medical College, Bengbu 233030, Anhui, China
*Corresponding author) |
| Abstract: |
| Objective To establish a rapid determination model based on near-infrared spectroscopy (NIRS) for glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian. Methods The contents of glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian from different places of origin were determined by high performance liquid chromatography (HPLC) as reference values. At the same time 2 200-2 049, 1 750-1 450, 1 151-1 001 nm and 1 795-1 475, 1 395-1 293, 1 125-1 030 nm wavelength ranges of near-infrared spectra were selected to establish the rapid determination model by combining partial least squares (PLS) regression analysis with cross validation method. Results The correlation coefficient and root-mean-squares error of cross validation of the established content calibration model were 0.980 and 0.184 for glycyrrhizic acid, and 0.919 and 0.144 for liquiritin, respectively. Conclusion The NIRS-PLS method is convenient, rapid and nondestructive for the content determination of glycyrrhizic acid and liquiritin for large number of Glycyrrhizae Radix et Rhizoma Yinpian, which provides a new and feasible method for the rapid quality evaluation of Glycyrrhizae Radix et Rhizoma Yinpian. |
| Key words: near-infrared spectroscopy Glycyrrhizae Radix et Rhizoma herbal pieces for decoction glycyrrhizic acid liquiritin rapid determination |
