| 摘要: |
| 目的 建立HPLC法同时测定五味子中2种三萜酸类成分甘五酸和黑老虎酸的含量，并考察不同产地五味子中甘五酸、黑老虎酸的含量差异，为提高药材质量研究提供依据。方法 色谱柱为Waters Symmetry C18柱（4.6 mm×250 mm，5 μm），流动相为甲醇-1%甲酸水（86∶14），检测波长220 nm，流速1.0 mL/min，柱温30℃，进样量10 μL。进行方法学考察，测定辽宁、吉林、湖北、湖南、陕西、山西、河南7个产地五味子样品中甘五酸、黑老虎酸的含量。运用SPSS软件（SPSSAU）对数据进行分析。结果 甘五酸、黑老虎酸在10～800 μg/mL范围内线性关系良好，相关系数均为0.999 7，平均加样回收率分别为97.25%（RSD=2.04%，n=6）和96.02%（RSD=2.03%，n=6）。不同产地五味子样品中的甘五酸和黑老虎酸含量变化较大。5个产地的南五味子（湖北、湖南、陕西、山西、河南产五味子）中甘五酸、黑老虎酸的含量均高于北五味子（辽宁、吉林产五味子），甘五酸含量最高的为河南产五味子［（0.285±0.015）mg/g］，最低的为吉林产五味子［（0.068±0.017）mg/g］；黑老虎酸含量最高的为山西产五味子［（0.927±0.017）mg/g］，最低为吉林产五味子［（0.039±0.010）mg/g］。结论 本研究建立的方法简单、准确、可靠、重复性好，适合同时对这2种三萜酸类成分甘五酸和黑老虎酸的定量分析。 |
| 关键词: 五味子属 三萜酸 甘五酸 黑老虎酸 高效液相色谱法 |
| DOI：10.16781/j.0258-879x.2021.01.0107 |
| 投稿时间：2020-05-12修订日期：2020-07-30 |
| 基金项目:国家自然科学基金（81573585），南京军区医学创新课题重大项目（14ZD02），海军军医大学（第二军医大学）本科学员创新实践孵化基地（FH2019076）. |
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| Determination of two triterpenoid acids in Schisandra sphenanthera and Schisandra chinensis from different habitats |
| GUO Liang-jun1,ZHENG Wei1,WANG Xiang1,JIA Lin2,CAI Meng-cheng3,JIN Yong-sheng4* |
(1. Department of Pharmacy, The 72nd Group Military Hospital of PLA Army, Huzhou 313000, Zhejiang, China;
2. Department of Pharmacy, Air Force Hospital of PLA Eastern Theater Command, Nanjing 210002, Jiangsu, China;
3. The First Student Team, College of Basic Medical Sciences, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
4. Department of Organic Chemistry, School of Pharmacy, Naval Medical University(Second Military Medical University), Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Objective To establish a high-performance liquid chromatography (HPLC) method for simultaneously determining the two triterpenoid acids (schisandronic acid[SA] and coccinic acid[CA]) in Schisandra, and investigate their content differences in Schisandra from different habitats, so as to provide basis for improving the quality of medicinal materials. Methods The HPLC condition was as follows:Waters Symmetry C18 column (4.6 mm×250 mm, 5 μm), the mobile phase was composed of methanol-0.1% formic acid solution (86:14), the detection wavelength was 220 nm, the flow rate was 1.0 mL/min, the column temperature was 30℃, and the injection volume was 10 μL. The contents of SA and CA were determined in Schisandra from Liaoning, Jilin, Hubei, Hunan, Shaanxi, Shanxi, and Henan. SPSS software (SPSSAU) was used to analyze the obtained data. Results There were good linear relationships in the range of 10-800 μg/mL for both SA and CA, the correlation coefficients were both 0.999 7, and the average recovery rates were 97.25% (RSD=2.04%, n=6) and 96.02% (RSD=2.03%, n=6), respectively. The contents of SA and CA in Schisandra from different habitats varied greatly. The contents of SA and CA in Schisandra sphenanthera (from Hubei, Hunan, Shaanxi, Shanxi, and Henan) were higher than those in Schisandra chinensis (from Liaoning and Jilin). The highest content of SA in Schisandra was from Henan ([0.285±0.015] mg/g) and the lowest one was from Jilin ([0.068±0.017] mg/g. The highest content of CA was from Shanxi ([0.927±0.017] mg/g) and the lowest one was from Jilin ([0.039±0.010] mg/g). Conclusion The method in this study is simple, accurate, reliable and reproducible, and it is suitable for the simultaneous quantitative analysis of the two triterpenoid acids SA and CA. |
| Key words: Schisandra triterpenoid acid schisandronic acid coccinic acid high-performance liquid chromatography |
