| 摘要: |
| 目的 建立一种无气管插管并不使用呼吸机通气的小鼠微创主动脉弓缩窄（TAC）技术。方法 随机将40只雄性2～4个月龄C57BL/6小鼠分为TAC手术组和假手术组，每组20只。TAC手术组小鼠用戊巴比妥钠溶液腹膜内注射麻醉，实施胸骨部分切开术后暴露主动脉弓，然后将缝线穿过主动脉弓下方并将主动脉弓系在钝的弯曲成90°的27号针上，完成结扎后拔出27号针。假手术组操作过程同TAC手术组，但不进行结扎。对比分析两组小鼠心脏质量/体重比、心肌肥大标志基因表达以及在体心脏功能。结果 与假手术组相比，TAC手术组小鼠在术后第28天心脏质量/体重比显著升高［（6.21±0.20）mg/g vs （3.94±0.19）mg/g，P＜0.01］，心肌肥大标志基因心房钠尿肽和脑钠肽表达升高（3.48±0.37 vs 1.00±0.12、3.92±0.41 vs 1.00±0.15，P均＜0.01），左心室射血分数和左心室短轴缩短率降低［（40.18±1.96）% vs （53.26±2.32）%、（23.12±2.08）% vs （32.16±2.64）%，P均＜0.01］。手术过程中及术后28 d内小鼠存活率为95.00%（38/40）。结论 微创TAC手术制备心肌肥厚模型小鼠的死亡率低，并能高效诱导心肌肥厚和降低心功能。 |
| 关键词: 主动脉弓缩窄 心肌肥厚 心力衰竭 动物模型 小鼠 |
| DOI：10.16781/j.CN31-2187/R.20210974 |
| 投稿时间：2021-09-26修订日期：2021-12-27 |
| 基金项目:国家自然科学基金（32071115，31471090）. |
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| Establishment of a mouse model of myocardial hypertrophy induced by transverse aortic constriction without ventilator support |
| FAN Juan,REN An-jing* |
(Experimental Teaching Center, College of Basic Medical Sciences, Naval Medical University (Second Military Medical University), Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Objective To establish a minimally invasive transverse aortic constriction (TAC) technique in mice without endotracheal intubation and ventilator ventilation. Methods Forty male C57BL/6 mice aged 2-4 months were randomly divided into TAC-operated group and sham-operated group, with 20 mice in each group. The mice in the TAC-operated group were anesthetized by intraperitoneal injection of sodium pentobarbital. After partial sternotomy, the aortic arch was exposed, and a suture was passed under the aortic arch and the aortic arch was tied to a blunt 90° curved 27-gauge needle, and then the needle was pulled out. The operation procedure of the sham-operated group was the same as that of the TAC-operated group, but no ligation was performed. The heart weight/body weight ratio, gene expression of cardiac hypertrophy markers and in vivo cardiac function of the 2 groups were compared and analyzed. Results Compared with the sham-operated group, the heart weight/body weight ratio of the mice was significantly increased on the 28th day after operation ([6.21±0.20]mg/g vs[3.94±0.19]mg/g, P<0.01), the expression of myocardial hypertrophy marker genes such as atrial natriuretic peptide and brain natriuretic peptide was significantly increased (3.48±0.37 vs 1.00±0.12, 3.92±0.41 vs 1.00±0.15, both P<0.01), and both left ventricular ejection fraction and left ventricular fraction shortening were significantly decreased in the TAC-operated group ([40.18±1.96]% vs[53.26±2.32]%,[23.12±2.08]% vs[32.16±2.64]%, both P<0.01). Furthermore, the survival rate was 95.00% (38/40) during surgery and within 28 d after surgery. Conclusion Minimally invasive TAC surgery can effectively induce myocardial hypertrophy and decrease cardiac function in mice, with low mortality. |
| Key words: transverse aortic constriction myocardial hypertrophy heart failure animal models mice |
