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| 严重急性呼吸综合征冠状病毒2受体血管紧张素转换酶2在人肺的表达 |
| 林裕贵1,2△,李培群1,2△,钟伟娟1,解中1,李周怡1,李程颐1,林春秀3,张增峰1,2* |
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(1. 广西医科大学基础医学院微生物学教研室, 南宁 530021;
2. 广西医科大学生物医药协同创新中心, 南宁 530021;
3. 江南大学食品科学与技术国家重点实验室, 无锡 214122
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 考察人肺中严重急性呼吸综合征冠状病毒2（SARS-CoV-2）受体血管紧张素转换酶2（ACE2）的表达，探讨ACE2在SARS-CoV-2感染中的意义。方法 利用基因型-组织表达（GTEx）数据库分析人体正常组织器官间ACE2的基因转录水平，免疫组织化学检测原代人肺组织中不同解剖部位ACE2的蛋白表达水平。结果 人肺中ACE2基因及蛋白表达水平均很低，主要表达于肺泡组织中的少量Ⅱ型肺泡上皮细胞，而在血管内皮组织以及支气管、细支气管、终末细支气管、呼吸性细支气管等各级支气管组织中均未见表达。结论 ACE2在人肺中表达水平很低，主要表达于少量Ⅱ型肺泡上皮细胞，表明人肺具有引起SARS-CoV-2感染的ACE2分子基础，SARS-CoV-2在人肺中可能存在其他感染机制。 |
| 关键词: 血管紧张素转换酶2 肺 严重急性呼吸综合征冠状病毒2 感染 受体 |
| DOI：10.16781/j.CN31-2187/R.20211124 |
| 投稿时间：2021-11-05修订日期：2022-04-29 |
| 基金项目:国家自然科学基金（31660041），广西壮族自治区自然科学基金（2018GXNSFAA281300）. |
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| Expression of a receptor of severe acute respiratory syndrome coronavirus 2 angiotensin-converting enzyme 2 in human lung |
| LIN Yu-gui1,2△,LI Pei-qun1,2△,ZHONG Wei-juan1,XIE Zhong1,LI Zhou-yi1,LI Cheng-yi1,LIN Chun-xiu3,ZHANG Zeng-feng1,2* |
(1. Department of Microbiology, College of Basic Medical Sciences, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
2. Collaborative Innovation Centre for Biomedicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
△Co-first authors.
* Corresponding author) |
| Abstract: |
| Objective To investigate the expression of angiotensin-converting enzyme 2 (ACE2) (a receptor of severe acute respiratory syndrome coronavirus 2[SARS-CoV-2]) in human lung and its significance in SARS-CoV-2 infection. Methods Genotype-Tissue Expression (GTEx) database was used to analyze the gene transcription levels of ACE2 in human normal tissues and organs. Immunohistochemistry was used to detect the protein expression levels of ACE2 in different anatomical sites of primary human lung tissues. Results The expression levels of ACE2 gene and protein in human lung were low, which were mainly expressed in a small number of type Ⅱ alveolar epithelial cells in alveolar tissue, but not in vascular endothelial tissue, bronchial, bronchiole, terminal bronchiole, respiratory bronchiole, or other bronchial tissues. Conclusion The expression of ACE2 in human lung is quite low, and is mainly located in a small number of type Ⅱ alveolar epithelial cells, indicating that human lung possesses the molecular basis for ACE2 to cause SARS-CoV-2 infection, and SARS-CoV-2 may have other mechanisms of infection in human lung. |
| Key words: angiotensin-converting enzyme 2 lung severe acute respiratory syndrome coronavirus 2 infection receptor |
