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| 新型氦氧潜水减压表安全性实验验证 |
| 周英杰1△,庆龙2△,朱包良1,衣洪杰3,王晔炜4,张坤1,徐伟刚1* |
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(1. 海军军医大学(第二军医大学)海军特色医学中心潜水与高气压医学研究室, 上海 200433;
2. 海军军医大学(第二军医大学)海军特色医学中心海潜科, 上海 200052;
3. 海军军医大学(第二军医大学)第一附属医院高压氧治疗科, 上海 200433;
4. 海军军医大学(第二军医大学)海军特色医学中心援潜救生医学与装备技术训练队, 上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 以猪模型验证新型氦氧潜水减压表的安全性，初步探索用动物实验结果评估潜水减压方案安全性的方法。方法 在专门建造的大动物加压舱内进行模拟潜水，按系统分层抽样法对常规作业深度每隔6 m、训练深度和例外暴露深度每隔9 m从新型氦氧潜水减压表中选取潜水方案进行验证，每个方案验证4～6次，观察模拟潜水期间动物的表现及减压后减压病症状、流经心脏的气泡，通过检测血液相关指标评估内皮损伤、炎症反应和氧化应激水平。结果 在全部14个验证方案中，所有动物均无氧中毒和减压病症状，出舱后未见循环气泡。在模拟潜水前和减压后即刻、6 h、24 h各时间点，动物的炎症反应指标（IL-1β、TNF-α）、内皮损伤指标（细胞间黏附分子1、血管细胞黏附分子1）和氧化应激指标（H2O2）差异均无统计学意义（P均＞0.05）。结论 就猪潜水模型而言，新型氦氧潜水减压表具有优良的安全性。本研究初步建立的实验评估潜水减压方案安全性的方法还需要更多的数据验证。 |
| 关键词: 氦氧潜水 减压病 减压方案 动物模型 减压表 |
| DOI：10.16781/j.CN31-2187/R.20220560 |
| 投稿时间：2022-07-02修订日期：2022-11-28 |
| 基金项目:军队后勤科研重点项目(BHJ19J007). |
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| Experimental verification on the safety of a newly-developed helium-oxygen diving decompression table |
| ZHOU Ying-jie1△,QING Long2△,ZHU Bao-liang1,YI Hong-jie3,WANG Ye-wei4,ZHANG Kun1,XU Wei-gang1* |
(1. Department of Diving and Hyperbaric Medical Research, Naval Medical Center, Naval Medical University (Second Military Medical University), Shanghai 200433, China;
2. Department of Naval Diving Medicine, Naval Medical Center, Naval Medical University (Second Military Medical University), Shanghai 200052, China;
3. Department of Hyperbaric Oxygen, The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200433, China;
4. Department of Submarine Rescue Training, Naval Medical Center, Naval Medical University (Second Military Medical University), Shanghai 200433, China
△Co-first authors.
* Corresponding author) |
| Abstract: |
| Objective To verify the safety of a newly-developed helium-oxygen diving decompression table in a swine model and preliminarily explore a method for evaluating the safety of decompression profiles based on animal experimental results. Methods Simulated diving was performed in a purpose-built pressurized chamber for large animals. The diving profiles were verified every 6 m at regular operation depth and every 9 m at training and exceptional exposure depth by systematic stratified sampling according to the newly-developed helium-oxygen diving decompression table, and each profile was verified 4-6 times. The animal conditions during simulated diving and symptoms of decompression sickness (DCS) and bubbles flowing through the heart after depression were observed. Blood indicators related to endothelial injury, inflammatory response and oxidative stress levels were assessed. Results No symptoms of oxygen toxicity or DCS, or circulating bubbles were found in the animals exposed with all the 14 tested profiles. The inflammatory response indexes (interleukin 1β, tumor necrosis factor α), endothelial injury indexes (intercellular adhesion molecule 1, vascular cell adhesion molecule 1) and oxidative stress index (H2O2) were compared before simulated diving and immediately (0 h), 6 h and 24 h after decompression, and there were no significant differences (all P＞0.05). Conclusion As far as the swine model is concerned, the newly-developed helium-oxygen diving decompression table has excellent safety. More data are needed to verify the preliminary experimental method for evaluating the safety of diving decompression table. |
| Key words: helium-oxygen diving decompression sickness decompression profiles animal models decompression table |
