| 摘要: |
| 单细胞多组学技术是指结合多种不同的生物学技术，对单个细胞进行多方面的分析和研究，从而获得更全面、更准确的单细胞数据。该技术包括单细胞基因组学、单细胞转录组学、单细胞蛋白质组学、单细胞表观组学等。单细胞多组学技术的发展为我们提供了一种更加精确理解生物体内复杂的细胞类型和功能的方式，尤其是对于异质性细胞群体中少数的特殊细胞类型如干细胞或罕见癌细胞，具有非常重要的应用价值。通过融合不同技术获得的信息，单细胞多组学可以更准确地描述单个细胞在多个生命事件和过程中的状态与变化，为生命科学的研究提供更加全面和深入的视角。本文系统概述了目前代表性单细胞多组学技术的发展现状，总结了其在生物学研究中的重要应用和巨大潜力。 |
| 关键词: 单细胞测序 单细胞多组学 基因组学 转录组学 蛋白质组学 表观组学 异质性 |
| DOI：10.16781/j.CN31-2187/R.20230446 |
| 投稿时间：2023-08-07修订日期：2023-12-13 |
| 基金项目: |
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| Single-cell multiomics technology and applications |
| ZHANG Jing,JIANG Yan,ZI Xiaoyuan* |
(Jiangsu Single-cell Sequencing Engineering Technology Research Center, Nanjing Engineering Research Center for High-Throughput Single-cell Sequencing, Nanjing 210016, Jiangsu, China
*Corresponding author) |
| Abstract: |
| Single-cell multiomics, involving various biological techniques for multifaceted analysis and research on a single cell to generate more comprehensive and accurate single-cell datasets, includes single-cell genomics, single-cell transcriptomics, single-cell proteomics, single-cell epigenetics, etc. Single-cell multiomics technology provides a more precise way to comprehend the complex cell types and functions, particularly for the more specialised cell types within the heterogeneous cell populations, such as stem cells or rare cancer cells, which have very important application value. By integrating information from different technologies, single-cell multiomics can more accurately characterise the state and changes of a single cell during multiple life events and processes, providing a more comprehensive and in-depth perspective for life science research. This article provides a systematic overview of the current representative single-cell multiomics technologies and summarizes their important application and potential in biological research. |
| Key words: single-cell sequencing single-cell multiomics genomics transcriptomics proteomics epigenetics heterogeneity |
