| 摘要: |
| 目的 探讨补肾活血方治疗子宫内膜异位症（EM）的免疫学机制。方法 通过将供体小鼠子宫内膜碎片注射入受体小鼠腹腔的方法构建EM小鼠模型，造模后将EM小鼠随机分为模型组、孕三烯酮组、补肾活血方组，每组8只。孕三烯酮组小鼠以孕三烯酮混悬液（0.325 mg/kg）灌胃、每周2次，补肾活血方组小鼠每日以补肾活血方中药液（43.68 g/kg）灌胃，疗程均为28 d。疗程结束后处死小鼠，通过大体标本和H-E染色观察小鼠异位病灶生长情况，采用Blauer粘连评分系统对小鼠腹腔粘连程度进行评分，采用免疫组织化学染色观察小鼠病灶纤维化标志物的表达，通过ELISA检测小鼠腹腔液中炎症因子水平，利用流式细胞术检测巨噬细胞极化变迁及巨噬细胞吞噬功能变化。结果 与模型组相比，补肾活血方组和孕三烯酮组EM小鼠异位病灶体积和腹腔粘连评分降低（均P＜0.05），病灶纤维化程度改善，腹腔液中TNF-α、TGF-β1、IL-12水平下调（均P＜0.05），腹腔巨噬细胞M2/M1比值降低（均P＜0.01），腹腔巨噬细胞的吞噬功能没有明显改变。补肾活血方组EM小鼠上述指标与孕三烯酮组相比无明显差异。结论 补肾活血方可能通过调控腹腔巨噬细胞改善小鼠腹腔免疫微环境治疗EM。 |
| 关键词: 子宫内膜异位症 巨噬细胞 免疫微环境 中医药 |
| DOI：10.16781/j.CN31-2187/R.20230297 |
| 投稿时间：2023-05-29修订日期：2023-09-04 |
| 基金项目:国家自然科学基金面上项目（82074206，81774352，81573755），上海市科学技术委员会科技创新行动计划（21Y21920500），海军军医大学（第二军医大学）第一附属医院“234学科攀峰计划”（2020YXK007），上海市中西医临床协作试点建设项目[ZY（2018-2020）-FWTX-1107]. |
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| Bushen Huoxue prescription regulates peritoneal macrophage for treating endometriosis in mice |
| BI Yanli,YU Chaoqin* |
(Department of Traditional Chinese Medicine (Gynecology), The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200433, China
* Corresponding author) |
| Abstract: |
| Objective To investigate the immunological mechanism of Bushen Huoxue prescription in the treatment of endometriosis (EM). Methods The EM mouse model was established by injecting endometrial fragments of donor mice into the abdominal cavity of recipient mice. EM mice were randomly divided into model group, gestrinone group and Bushen Huoxue prescription group, with 8 mice in each group. The mice in gestrinone group were intragastrically administered with gestrinone suspension (0.325 mg/kg) twice a week, while the mice in Bushen Huoxue prescription group were intragastrically administered with Bushen Huoxue prescription (43.68 g/kg) daily for 28 d. After treatment, the mice were sacrificed. The growth of ectopic lesions in mice was observed by gross specimens and hematoxylin-eosin staining. The degree of abdominal adhesion in mice was scored by Blauer adhesion scoring system. The expression of fibrotic markers in mouse lesions was observed by immunohistochemical staining. The levels of inflammatory factors in mouse peritoneal fluid were detected by enzyme-linked immunosorbent assay. The changes of macrophage polarization and phagocytosis were detected by flow cytometry. Results Compared with the model group, the volume of ectopic lesions and abdominal adhesion score of EM mice in the Bushen Huoxue prescription group and gestrinone group were significantly decreased (all P＜0.05), the degree of lesion fibrosis was improved, the levels of tumor necrosis factor-α, transforming growth factor-β1 and interleukin-12 in peritoneal fluid were significantly decreased (all P＜0.05), the M2/M1 ratio of peritoneal macrophages was significantly decreased (both P＜0.01), and the phagocytic function of peritoneal macrophages had no significant change. The above indexes in the Bushen Huoxue prescription group had no significant difference compared with those in the gestrinone group. Conclusion Bushen Huoxue prescription can improve the immune microenvironment by regulating peritoneal macrophages for treating EM in mice. |
| Key words: endometriosis macrophage immune microenvironment traditional Chinese medicine |
