| 摘要: |
| 目的：探讨脊髓伤后早期伤段脊髓组织一氧化氮合酶(NOS)活性动态变化的规律及其组织细胞来源。方法：检测伤段脊髓组织中3H标记的精氨酸转化生成3H-瓜氨酸的量，了解脊髓压迫伤后0-60min伤段脊髓组织NOS的活性变化；并应用免疫组织化学方法研究其细胞定位。结果：脊髓压迫伤后，NOS活性明显增加，伤后5min达最高点，后又迅速下降，于伤后1h恢复至正常水平。免疫组化可见脊髓灰质中间外侧细胞柱、中央管周围及背角和腹侧角有大量的NOS-Ⅰ型阳性细胞，而未见NOS-Ⅲ型阳性细胞。结论：在脊髓伤后1h内，伤段脊髓组织NOS活性迅速而短暂升高；其来源可能主要是脊髓灰质的神经细胞。 |
| 关键词: 脊髓损伤 一氧化氮合酶 一氧化氮 |
| DOI： |
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| 基金项目: |
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| Dynamical changes of nitric oxide synthase activity in injured spinal cord tissue after spinal cord injury and its cell derivation |
| 张春才,侯铁胜,马大烈,靳安民,李明,周初松 |
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| Abstract: |
| Objective: To explore dynamical changes of nitric oxide synthase (NOS) activity in damaged spinal cord tissue at early phase of spinal cord injury and its cell derivation. Methods: NOS activity was determined by the rate of conversion of 3H-arginine to 3H-citrulline in damaged spinal cord tissue from 0 to 60 min after spinal cord compression, and immunohistochemistry was applied to study NOS derivation. Results: The NOS activity increased sharply after spinal cord compression injury, reached the highest at 5 min, and then decreased rapidly to normal level in 1 h. NOS-Ⅰ immunoreactive interneurons were identified mainly in the intermediolateral cell column, around the central canal and in dorsal and ventral horn, but there were no cells with NOS-Ⅲ immunoreaction in spinal cord tissue. Conclusion: There is a sharp transient increase in NOS activity during the first hour of spinal cord compression, the derivation is mainly interneurons in spinal cord. |
| Key words: spinal cord injuries nitric oxide synthase nitric oxide |
