| 摘要: |
| 目的：研究白细胞介素6(IL-6)和肿瘤坏死因子(TNF)对人脐静脉内皮细胞（HVEC）的损伤作用。方法：以原代培养的HVEC为模型，将IL-6、TNF及其抗体与HVEC共同孵育，观察内皮细胞（EC）形态变化和培养液中血管紧张素转化酶（ACE）和乳酸脱氢酶（LDH）水平。结果： TNF和IL-6可引起细胞间隙增大，甚至细胞脱落；并且随着TNF和IL-6浓度加大及作用时间延长，培养液中ACE和LDH水平升高。结论： IL-6和TNF对血管EC有损伤作用。 |
| 关键词: 内皮，血管 肿瘤坏死因子 白细胞介素6 |
| DOI： |
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| 基金项目: |
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| Effects of interleukin-6 and tumor necrosis factor on cultured vascular endothelial cells |
| 丁思奇,吴宗贵,侯健,张国元,马丽萍 |
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| Abstract: |
| Objective： To study the damaging effects of interleukin-6 (IL-6) and tumor necrosis factor (TNF) on human vascular endothelial cells (HVEC). Methods: A HVEC model was developed. After being incubated with IL-6, TNF and their antibody, HVEC morphological changes were observed, and the levels of lactate dehydrogenase (LDH) and angiotensin converting enzyme (ACE) in the culture fuild were measured. Results: TNF and IL-6 could cause enlargement in intercellular space, even the exfoliation of HVEC, moreover, ACE and LDH levels increased in a time- and dose-dependent manner. Conclusion: IL-6 and TNF have damaging effects on HVEC. |
| Key words: endothelium, vascular tumor necrosis factor interleukin-6 |
