| 摘要: |
| 目的：以烧伤小鼠为模型研究供体皮肤特异性延长成活的可行性。方法：C57BL/6小鼠造成20％TBSAⅢ度烧伤创面，48h后切痂，移植BALB/c或C3H/He小鼠皮肤，经60Co射线全身照射9Gy，立即注射无T淋巴细胞的C57BL/6小鼠和BALB/c小鼠的骨髓细胞。组Ⅰ为烧伤的C57BL/6小鼠移植BALB/c小鼠皮肤；组Ⅱ在组Ⅰ基础上行放射处理；组Ⅲ在组Ⅱ基础上行混合骨髓移植；组Ⅳ类似组Ⅲ，但移植的皮肤为C3H/He；组Ⅴ为C57BL/6小鼠同系皮肤移植对照组。结果：除组Ⅱ小鼠由于骨髓耗竭而全部死亡外，其他各组间动物死亡率无差异，未见移植物抗宿主病表现。异体皮平均成活时间组Ⅰ为(8.4±1.5)d；组Ⅱ(17.5±3.7)d；组Ⅳ(32.5±8.1)d；组Ⅲ为(78.2±5.6)d，与组Ⅰ、组Ⅱ和组Ⅳ比较P<0.05；组Ⅴ90d。结论：C57BL/6小鼠对BALB/c小鼠皮肤显示了特异性移植耐受，而排斥MHC不相关的C3H/He的小鼠皮肤。本研究也为进一步的临床基础研究奠定了基础。 |
| 关键词: 烧伤 皮肤移植 放射耐受 |
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| Donor-specific unresponsiveness to skin allografts in burned mouse |
| 刘世康,陈玉林,夏照凡,韦多,朱世辉,唐洪泰,苏波 |
| () |
| Abstract: |
| Objective: To provide a permanent wound coverage by using skin allografts in mouse model with fullthickness burn of 20%TBSA. Methods: The recipient mouse was treated with 9 Gy total body irradiation from 60Co source, followed by infusion of a mixed donor and recipient T-cell depleted bone marrow where a state of specific unresponsiveness to the skin allograft was induced without the need for chronic immunosuppression. Five experimental groups were divided: group Ⅰ, 20%TBSA burn of C57B/6 mice with skin allograft from a BALB/c mouse at 48 h without irradiation; group Ⅱ, similar to group Ⅰ but with irradiation; group Ⅲ, similar to group Ⅱ but with the infusion of mixed bone marrow; group Ⅳ, similar to group Ⅲ but with skin allograft from a C3H/He mouse; group Ⅴ, controlled C57BL/6 mice with homograft. Results: There were no statistical difference irranimal mortality in all groups except group Ⅱ, and no evidence of infectious morbidity and graft vs host disease were found. Mean skin allograft survival (mice died with intact graft were excluding) was as follows: group Ⅰ, (8.4±1.5) d; group Ⅱ, (17.5±3.7) d; group Ⅳ, (32.5±8.1) d, and group Ⅴ, 90 d; gorup Ⅲ, (78.2±5.6) d (P<0.05 vs group Ⅰ, Ⅱ and Ⅳ). Conclusion: C57BL/c mice exhibited specific transplantation unresponsiveness to the BALB/c mouse skin, but rejected the third party MHC-disparate C3H/He mouse skin. This study suggests the potential use of induced specific unresponsiveness to skin allografts for wound coverage in thermal injury. |
| Key words: burns allograft irradiation tolerance |
