| 摘要: |
| 目的:研究人肾细胞癌(RCC)原代细胞生物学特性及其癌基因的表达情况。方法：应用改良的原代肿瘤细胞分离法分离培养7例人RCC原代细胞。应用染色体核型分析和免疫组化方法，分析原代细胞核型以及癌基因与抑癌基因的表达。结果：用本法培养的原代细胞比其他常规方法所得细胞既多且纯。所培养的人RCC原代细胞的癌基因表达分析发现bcl-2，c-erbB-2，c-met，表皮生长因子受体(EGFR)有轻度至中度表达,而p53没有表达。结论：人RCC原代培养及其癌基因表达检测,为应用原代RCC所开展exvivo的RCC基因治疗打下了初步的基础。 |
| 关键词: 肾肿瘤 原代培养 癌基因 |
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| Preliminary analysis of primarily cultured cells of human renal cell carcinoma and expression of the oncogenes |
| 戚中田,侯建国,曹广文,张晓琴 |
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| Abstract: |
| Objective: To study the characteristic of primary cells of human renal cell carcinoma(RCC) and expression of the oncogenes. Methods: An improved isolation method was employed to isolate primary RCC cells from 7 fresh human RCC specimens. The expression of some oncogenes in the cells was examined by means of chromosomal pattern analysis and immunohistochemistry. Results:It was found that the primary cells obtained by using this met hod was more and purer than that of the routine methods and bcl-2, c-erbB-2 , c-met, EGFR had low to medium level expression, but p53 had no expression. Conclusion: The study provides a basis for the ex vivo gene th erapy for RCC. |
| Key words: kidney neoplasms primary cell culture oncogenes |
