| 摘要: |
| 目的：研究小鼠脾脏是否存在褪黑素(M)结合位点，该结合位点是否存在昼夜节律。方法：应用放射配体结合法检测小鼠脾脏M结合位点（MR）。每4 h处死一批动物，研究不同时间脾脏MR的差异；分别持续光照及注射M 21 d，观察MR结合量及昼夜节律的改变。结果：小鼠脾脏存在MR，其特点为低结合容量，高亲和力，对M具高度特异性，符合特异结合位点的基本条件；昼夜节律研究显示，MR结合量峰值在12:00-16:00，谷值在0:00-4:00，F检验峰谷值间差异显著(P<0.01)。结论：小鼠脾脏存在MR，其MR有昼夜节律变化；持续光照及注射M不影响MR节律，表明MR昼夜节律的机制是自主的，具生理学意义。 |
| 关键词: 褪黑素、结合位点 脾脏 昼夜节律 |
| DOI： |
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| 基金项目: |
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| Circadian rhythm of melatonin receptor in the mouse spleen |
| 彭树勋,邵福源,赵瑛 |
| () |
| Abstract: |
| Objective: To determine whether there exists melatonin receptor (MR) in the mouse spleen and the circadian rhythm (CR) in MR. Methods: Specific binding of melatonin to the mouse spleen was measured by radioligand binding assay. Results: The results revealed the maximum binding capacity (Bmax) was (1.25±0.21) fmol/mg protein and equilibrium constant (Kd)was (59.5±10.1) pmol/L. The peak values of Bmax of MR was from 12:00 to16:00 and the trough values was between 0:00 to 4:00. The difference between them was significant statistically. CR of MR showed no change when continued light (3 weeks) and injected melatonin in peritoneum (3 weeks). Conclusion: MR is present in the mouse spleen and there exists CR of MR in the mouse spleen. It is indicated that CR of MR in spleen is independent from that of melatonin. |
| Key words: melatonin binding site spleen circadian rhythm |
