| 摘要: |
| 目的：建立体外大量扩增树突状细胞(DC)的方法,并对DC进行形态学和免疫学性质鉴定。方法：制备小鼠骨髓细胞，用免疫磁珠方法去除其中的淋巴细胞、单核巨噬细胞及粒细胞，将阴性选择到的细胞用小鼠重组GM-CSF培养，8 d后收集悬浮细胞。将收集到的细胞进行免疫表型鉴定和超微结构观察，以及DC特异性抗原DEC205的免疫胶体金标记。结果：可从每只小鼠的股骨骨髓中扩增到(2-5)×106 个DC, 细胞纯度达80％以上。细胞表达DC的特异性抗原DEC205，具有DC的典型形态及表型。结论：成功地建立了体外大量扩增骨髓DC的方法，为深入研究其免疫学功能打下基础。 |
| 关键词: 树突状细胞 超微结构 |
| DOI： |
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| 基金项目: |
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| Amplification and ultrastructure observation of murine bone marrow-derived dendritic cells |
| 郑尊,于秉学,颜永碧,章卫平,曹雪涛,谢志芳 |
| () |
| Abstract: |
| Objective: To amplify large number of dendritic cells (DCs) from murine bone marrow and assay their morphological, immunological characteristics. Methods: Lymphocytes, monocytes-macrophages and granulocytes of murine bone marrow were depleted using immuno-Minimagnetic cell sorter. The selected cells were cultured with granulocyte-macrophage colony-stimulating factor, and the floating cells were collected 8 d after culture. The phenotype of collected DCs was analyzed, and their morphology was observed under electron microscopy. Results: (2-5)×106 DCs were obtained from bone marrow of one mice, and the purity of DCs was more than 80%. The cells expressed specific antigens (DEC205) of DCs and had typical morphology of DCs. Conclusion: A method to generate large number of DCs from murine bone marrow is established, which contribute to further studying immunological functions of DCs. |
| Key words: dendritic cells ultrastructure |
