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| HPLC-ELSD法测定天冬总呋甾皂苷中AR-Ⅰ的含量 |
| 徐从立,陈海生,谭兴起,宣伟东,梁爽,XUCong-li,CHENHai-sheng,TANXing-qi,XUANWei-dong,LIANGShuang |
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| 摘要: |
| 目的:测定天冬总呋甾皂苷中AR-Ⅰ的含量.方法:采用高效液相色谱法,色谱条件:Diamonsil C18(5 mm×250mm,5 μm)色谱柱;流动相:CH 3CN-H2O(36:64);流速:0.8 ml·min1;进样量20μl;柱温:室温.以ELSD为检测器,气化温度40℃,气体(N2)压力2.0×105Pa.结果:AR Ⅰ在0.094～0.940 0 g·L-1范围内呈良好线性关系(r=0.999 2),检测具有较高的灵敏度和稳定性,平均回收率为101.3％(n=6),RSD=2.36％.结论:HPLC ELSD为天冬呋甾皂苷的测定提供了简便实用的分析方法. |
| 关键词: 天冬总呋甾皂苷、AR-Ⅰ、色谱法,高效液相 |
| DOI：10.3724/SP.J.1008.2006.00427 |
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| 基金项目:国家自然科学基金（20472113）. |
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| HPLC-ELSD in determination of AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis |
| 徐从立,陈海生,谭兴起,宣伟东,梁爽,XU Cong-li,CHEN Hai-sheng,TAN Xing-qi,XUAN Wei-dong,LIANG Shuang |
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| Abstract: |
| Objective： To determine AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis. Methods： High performance liquid chromatography （HPLC） was used and the condition was： Diamonsil C18 column （5 mm×250 mm, 5μm）, mobile phase： CH3CN-H2O （36 ： 64） ：flow rate： 0.8 ml/min; injector volume：20μl;colum temperature： room temperature. Evaporative light scattering detector（ELSD） condition was： drift tube temperature： 40℃, and nebulizer gas pressure （N2） ： 2.0×10^5 Pa. Results： The linearity was good when AR-I was within the range of 0. 094-0. 940 0 g.L^-1 （r=0. 999 2）. The test had higher sensitivity and good stability. The average recovery was 101.3% （n= 6）and RSD was 2.36%. Conclusion： HPLC-ELSD is practical and reliable for the determination of the furostanol saponins of Asparagus cochinchinensis |
| Key words: total furostanol saponins of Asparagus cochinchinensis AR-Ⅰ chromatography, high pressure liquid |
