| 摘要: |
| 目的:为降低手术难度,设计一个能客观反映大鼠肝移植胆道缺血再灌注损伤的模型.方法:采用80只SD大鼠建立此模型,并使用门静脉、腹主动脉双重恒压灌注,除血管吻合外,其余方法同大鼠原位肝移植.且门静脉、肝动脉再灌注由血管夹控制.结果:本模型手术成功率95％(76/80),热、冷缺血和再灌注时间可精确控制,无肝期(16±2)min.双重恒压灌注效果好,光镜下胆道壁毛细血管未见红细胞.结论:本模型模拟了肝移植的全过程,手术简单、成功率高、可比性强.且排除了免疫因素对胆道损伤的影响,更好的反映了胆道缺血再灌注损伤的病理生理过程,为肝移植胆道损伤的基础研究提供了一种新的实验方法. |
| 关键词: 肝移植、胆道、再灌注损伤、模型,动物 |
| DOI：10.3724/SP.J.1008.2006.00429 |
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| 基金项目: |
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| Establishment of an orthotopic autologous liver transplantation model with bile ducts ischemia-reperfusion injury in rats |
| 赵宏峰,周杰,ZHAO Hong-feng,ZHOU Jie |
| () |
| Abstract: |
| Objective：To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats, so as to make it easy for the operation. Methods： Eighty SD rats were used to establish the models. The method used for this model was the same as the one for orthotopic liver transplantation, except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta. The portal vein and the hepatic artery reperfusion were controlled by artery clamp. Results： The successful rate of the operation for establishing the model was 95 % （76/80） and the anhepatic phase was （16±2） min. The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good. There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion. Conclusion： This technique, simulating the whole process of liver transplantation, is simple and has high successful rate. Besides,it rules out the influence of immune agent on bite ducts impairment, and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury, providing a novel approach for investigation of bile ducts injury during liver transplantation |
| Key words: liver transplantation biliary tract reperfusion injury models, animal |
