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| 大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化 |
| 周运恒,缪明永,李洋,时多,黄鹤,王学敏,焦炳华,ZHOUYun-heng,MIAOMing-yong,LIYang,SHIDuo,HUANGHe,WANGXue-min,JIAOBing-hua |
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| 摘要: |
| 目的:研究大鼠肝再生过程中肝细胞caspase-3和caspase-8活性的变化规律.方法:雄性SD大鼠70％肝切除(PH),制作肝再生模型,制备肝匀浆液,经离心后用酶标仪分别测定肝再生过程中不同时期(共8个时间点),肝细胞的凋亡酶活性.结果:Caspase-3活性,与对照组相比,在PH后3、6、72、120 和168 h时显著性增高(P<0.001);而整个过程中Caspase-8的活性除了在PH后168 h时高于对照组外(P<0.05),其余时间点没有明显增高.结论:肝再生过程中肝细胞凋亡酶Caspase-3活性的升高可能主要是由内源性线粒体途径引起的. |
| 关键词: 肝再生、肝细胞、caspase-3、caspase-8 |
| DOI：10.3724/SP.J.1008.2006.00500 |
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| 基金项目:国家自然科学基金（30070346）. |
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| Changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats |
| 周运恒,缪明永,李洋,时多,黄鹤,王学敏,焦炳华,ZHOU Yun-heng,MIAO Ming-yong,LI Yang,SHI Duo,HUANG He,WANG Xue-min,JIAO Bing-hua |
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| Abstract: |
| Objective：To study the changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats. Methods： Liver regeneration model was produced by 70% partial hepatectomy （PH） in male SD rats. Then the rats were sacrificed and the liver homogenate were prepared. Hepatic caspase-3 and caspase-8 activities were determined at 8 different time points during the liver regeneration at the absorbance of 405 nm. Results： Hepatic caspase-3 activity increased significantly 3 h, 6,72 h, 120 h and 168 h after PH compared with that of control group（P〈0. 001）; Hepatic caspase-8 activity increased 168 h after PH（P%0.05） and had no obvious change at any other time points. Conclusion： Our result suggests that the increase of caspase-3 activity during the liver regeneration may be mainly induced by the mitochondrial pathway. |
| Key words: liver regeneration hepatocytes caspase-3 caspase-8 |
