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| 人参皂苷、丹参酮和川芎嗪抗小鼠皮肤衰老作用研究 |
| 王红丽,吴铁,吴志华,陈垦,郭其杰,WANGHong-li,WUTie,WUZhi-hua,CHENKen,GUOQi-jie |
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| 摘要: |
| 目的:比较人参皂苷、丹参酮和川芎嗪灌服对衰老小鼠皮肤的抗衰老作用.方法:100只雌性昆明种小鼠,随机分为5组(n=20):正常对照组皮下注射生理盐水;其余4组每日颈背部皮下注射D-半乳糖(1 000 mg·kg-1·d-1)造成小鼠衰老模型,同时各组分别灌胃生理盐水(衰老模型组)、丹参酮(1 500 mg·kg-1·d-1)、川芎嗪(150 mg·kg-1·d-1)或人参皂苷(100 mg·kg-1·d-1).42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶(SOD)活力、过氧化脂质代谢产物丙二醛(MDA)含量、过氧化氢酶(CAT)活力和皮肤羟脯氨酸含量.结果:灌服丹参酮和人参皂苷后,衰老模型小鼠皮肤中SOD活力由衰老对照组的(131.2±21.5) U/ml分别提升为(203.1±11.2) U/ml和(196.8±27.5) U/ml,羟脯氨酸含量由(0.57±0.13) mg/g分别提高到(0.71±0.11) mg/g和(0.76±0.12) mg/g,MDA含量由(9.39±1.5) nmol/g降低到(6.4±1.3) nmol/g和(7.16±2.3) nmol/g,差异均有显著性(P<0.05),且丹参酮和人参皂苷的效果相当.而灌服川芎嗪组上述指标的改变不显著.结论:人参皂苷和丹参酮灌服有显著的抗小鼠皮肤衰老作用,均可明显提高皮肤抗氧化酶活力和增强成纤维细胞活性;川芎嗪灌服抗小鼠皮肤衰老作用不明显. |
| 关键词: 皮肤衰老、人参皂苷、丹参酮、川芎嗪 |
| DOI：10.3724/SP.J.1008.2006.00525 |
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| Anti-aging effects of ginsenosides, tanshinone and chuanxionyzine on mice skin: a comparison study |
| 王红丽,吴铁,吴志华,陈垦,郭其杰,WANG Hong-li,WU Tie,WU Zhi-hua,CHEN Ken,GUO Qi-jie |
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| Abstract: |
| Objective：To compare the anti-aging effects of ginsenosides,tanshinone and chuanxionyzine on the skin of D-galactose-induced mice aging model. Methods.. One hundred female mice were randomly d[vided into 5 groups（n= 20）. Mice in normal control group received subcutaneous injection of normal saline and mice in other 4 groups received cervicodorsal injection of D-galactose （1 000 mg·kg^-1·d^-1 ） for 42 d to establish subacute aging model. The experimental groups included aging model group （without further treatment）, tanshinone-treated （1 500 mg·kg^-1·d^-1） group,chuanxionyzine-treated （150 mg·kg^-1·d^-1） group,and ginsenosides-treated （100 mg·kg^-1·d^-1） group. Forty two days later,the dorsal skin samples were collected to determine SOD,CAT activities and MDA,hydroxyproline contents. Results： In tanshinone- and ginsenosides-treated groups,SOD activities increased from （131.2±21.5） U/ml to （203.1 ±11.2） U/ml and （196.8±27.5） U/ml,respectively; the contents of hydroxyprolin increased from （0.57±0.13） mg/g to （0.71±0.11） mg/g and （0.76±0.12） mg/g,respectively; and the contents of MDA decreased from （9.39± 1.5） nmol/g to （6.4±1.3） nmol/g and （7.16 ± 2.3） nmol/g, respectively （all P〈0.05）. However, there was no significant difference between the parameters of tanshinone- and ginsenosides-treated groups. Compared with aging control group, there was no obvious changes in the above parameters in chuanxionyzine-treated group. Conclusion： Both ginsenosides [100 mg/（kg ·d） ]and tanshinone [1 500 mg/（kg ± d）] have noticeable anti-skin aging effects via i.g. administration through improving the activities of antioxidant enzyme and dermal fibroblasts. Chuanxionyzine [150 mg/（kg · d）] shows no anti-skin aging effects in mouse aging models. |
| Key words: skin aging ginsenosides tanshinone chuanxionyzine |
