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炮制前后知母中芒果苷和新芒果苷的含量变化
刘敏,赵白云,赵亮,娄子洋,柴逸峰,LIUMin,ZHAOBai-yun,ZHAOLiang,LOUZi-yang,CHAIYi-feng
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摘要:
目的:考察炮制前后知母(Anemarrhena asphodeioides Bge.)中黄酮类成分芒果苷和新芒果苷的含量变化.方法:采用HPLC法测定知母、炒知母中芒果苷和新芒果苷的含量,流动相为乙腈-25 mmol·L-1磷酸二氢钾缓冲液(pH 3.0)梯度洗脱.应用中药色谱指纹图谱相似度评价系统2004 A版(国家药典委员会)对3个批号的知母炮制前后的色谱图进行评价.结果:3个批号的知母炮制前后芒果苷的含量升高,而新芒果苷的含量下降;炮制前后图谱的相似度均有所下降(<1).结论:炮制后知母中黄酮类成分芒果苷和新芒果苷的含量发生了变化.
关键词:  炮制、知母、芒果苷、新芒果苷
DOI:10.3724/SP.J.1008.2006.00528
基金项目:上海市科委科技攻关(02419125);上海市科委重大项目(2403DZ19548).
Changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing
刘敏,赵白云,赵亮,娄子洋,柴逸峰,LIU Min,ZHAO Bai-yun,ZHAO Liang,LOU Zi-yang,CHAI Yi-feng
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Abstract:
Objective:To determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. Methods: High performance liquid chromatography (HPLC) was used to determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. The mobile phase was ACN: 25 mmol/L KH2 PO4 (pH 3.0), gradient elution. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A edition) was used to evaluate the similarity of the 3 batches of Rhizoma Anemarrhenae before and after processing. Results: Mangiferin in the 3 batches was upregulated and neomangiferin was downregulated afer prosessing. The similarity of the 3 batches was all decreased(〈 1). Conclusion: Processing changes the contents of mangiferin and neomangiferin in Rhizoma Anernarrhenae.
Key words:  processing  anemarrhenae  mangiferin  neomangiferin