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天冬中呋甾皂苷含量测定方法的建立
沈阳,陈海生,徐从立,徐红利,SHENYang,CHENHai-sheng,XUCong-li,XUHong-li
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摘要:
目的:采用E试剂比色法建立了天冬总皂苷含量测定方法.方法:用对二甲氨基苯甲醛-HCl乙醇液的发色体系,以天冬皂苷AC-9作为指标,在波长510 nm测定天冬总皂苷含量.结果:标准曲线在0.08~0.40 mg范围内线性关系良好,平均回收率为99.79%(RSD=1.05%).结论:此法可作为评价天冬总皂苷的质量标准.
关键词:  天冬、呋甾皂苷、E试剂、分光光度法
DOI:10.3724/SP.J.1008.2006.00548
基金项目:国家自然科学基金(20472113).
A method for determination of furostanol saponins in saponins extract from Radix Asparagi
沈阳,陈海生,徐从立,徐红利,SHEN Yang,CHEN Hai-sheng,XU Cong-li,XU Hong-li
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Abstract:
Objective:To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in saponins extract from Radix Asparagi. Methods: Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde, a colorimetry method was established to determine the content of furostanol saponins in saponins extract from Radix Asparagi at 510 nm. Saponins AC- 9 was measured as reference in the determination. Results: The standard curve of saponins AC-9 showed good linearity in the range of 0.08-0.40 mg and the average recovery was 99.79% (RSD= 1, 05% ). Conclusion.. This method is rapid, accurate and easy to be carried out, and it can be used to control the quality of Radix Asparagi
Key words:  Radix Asparagi  furostanol saponirs  Ehrlich  spectrophotmetry