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| 肝素-ELISA法检测血清中期因子及其对肝癌诊断的初步应用 |
| 李瑾,钱海华,康晓燕,杨之斌,吴孟超,殷正丰,LIJin,QIANHai-hua,KANGXiao-yan,YANGZhi-bin,WUMeng-chao,YINZheng-feng |
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| 摘要: |
| 目的:建立中期因子(midkine,MK)的测定方法,初步探讨血清MK浓度测定对肝癌的诊断价值.方法:根据MK可结合肝素的特点,应用肝素-抗体双夹心原理,建立肝素-ELISA,并检测20例健康献血者、20例良性肝占位性病变患者、20例肝硬化患者以及104例肝细胞癌(HCC)患者血清MK的质量浓度.结果:肝素-ELISA检测MK的最小值为15 ng/L,可测范围为15～1 000 ng/L,＜500 ng/L时呈线性关系(r=0.97).测定结果表明,正常献血者、良性肝占位性病变患者和肝硬化患者血清MK浓度分别为(56±11)、(74±19)和(89±43)ng/L,而HCC患者血清MK浓度为(199±87)ng/L,与前3组之间有显著差异(P＜0.05).HCC患者血清MK阳性率达83.7％,与其他3组之间具有显著性差异(P＜0.001).结论:应用肝素-ELISA测定血清MK的质量浓度对肝癌具有诊断价值. |
| 关键词: 肝素、酶联免疫吸附、中期因子、肝细胞癌 |
| DOI：10.3724/SP.J.1008.2006.00631 |
| 投稿时间：2006-02-27修订日期：2006-04-10 |
| 基金项目:国家自然科学基金（30400531）;国家“973”计划项目（2002CB513100）. |
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| Heparin-enzyme linked immunosorbent assay in determination of serum midkine concentration and its application in diagnosis of hepatocellular carcinoma |
| 李瑾,钱海华,康晓燕,杨之斌,吴孟超,殷正丰,LI Jin,QIAN Hai-hua,KANG Xiao-yan,YANG Zhi-bin,WU Meng-chao,YIN Zheng-feng |
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| Abstract: |
| Objective： To develop a method for determination of serum midkine （MK） concentration and to evaluate its value in the diagnosis of hepatocellular carcinoma （HCC）. Methods： Since heparin can bind to MK, a heparin-enzyme linked immunosorbent assay （ELISA） for determination of MK was developed using heparin-antibody sandwich technique. Serum MK levels were measured in 20 healthy adults, 20 patients with benign liver tumors, 20 with liver cirrhosis,and 104 with HCC. Results： The method had a minimum detectable concentration of 15 ng/L and a detectable range of （15-1 000） rig/L, and a linearity was found when the concentration was 〈500 ng/L（r=0.97）. Serum MK levels of the healthy subjects,the patients with benign liver tumor, liver cirrhosis and HCC were （56±11） rig/L,（74±19） ng/L, （89±43） ng/L and （199±87） ng/L, respectively; with significant difference found between HCC group and the former 3 groups （P〈0.05）. The positive rate of MK in HCC group was 83.7% ,significantly higher than those of 3 other groups （P〈0. 001）. Conclusion： The heparin-ELISA method for MK determination has diagnostic value for HCC. |
| Key words: heparin enzyme linked immunosorbent assay midkine hepatocellular carcinoma |
