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| 皮肤组织黑素细胞与胶原和弹力纤维的组合染色法研究 |
| 张顺民,龚志锦,陈伟红,郑建明,高莉,ZHENGJian-ming,GAOLi,ZHANGShun-min,GONGZhi-jin,CHENWei-hong |
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| 摘要: |
| 目的:寻找能显示皮肤组织黑素细胞与胶原纤维和弹力纤维的染色法.方法:选用Masson Fontana silver nitrate-Ponceau S-Picric acid-Victoria blue(Masson Fontana硝酸银、丽春红S、苦味酸和维多利亚蓝,简称MFSN-P-PA-VB)对实验动物皮肤组织进行组合染色.结果:皮肤黑素细胞颗粒呈黑色;胶原纤维呈红色,出血的红细胞呈黄色;弹力纤维呈绿色.结论:本组合染色方法克服了原法染色单一、对比效果较差的弱点.能够较好的显示皮肤组织中含有黑素细胞颗粒、胶原纤维及出血部位的红细胞和弹力纤维组织成分. |
| 关键词: 组合染色法、皮肤、黑素细胞、胶原纤维、弹力纤维 |
| DOI：10.3724/SP.J.1008.2006.00670 |
| 投稿时间：2005-11-25修订日期：2006-04-26 |
| 基金项目: |
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| A compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues |
| 张顺民,龚志锦,陈伟红,郑建明,高莉,ZHENG Jian-ming,GAO Li,ZHANG Shun-min,GONG Zhi-jin,CHEN Wei-hong |
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| Abstract: |
| Objective：To develop a compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues. Methods： A staining method combining Masson Fontana silver nitrate, Ponceau S, Picric acid and Victoria blue （MFSNP-PA-VB） was used in the present animal skin experimental. Results： The melanin granule was black in the skin melanocyte, collagenic fibers were red, the red blood cells at hemorrhagic focus were yellow, and the elastic fibers were green by this MFSNP-PA-VB staining method. Conclusion： This compound staining method has multicolor and fine contrast, and can better show melanins, the red blood cells at hemorrhagic focus, and collagenic and elastic fibers |
| Key words: compound staining method skin melanocyte collagenic fiber elastic fiber |
