| 摘要: |
| 目的:构建慢性肾衰竭继发甲状旁腺功能亢进大鼠模型,为研究肾性甲旁亢的发病机制及治疗奠定基础.方法:36只8～10周龄健康雄性SD大鼠,体质量200～250 g,随机分为6组(n=6):5/6肾切除高磷饲料组(STNx+HP);5/6肾切除低磷饲料组(STNx+LP);5/6肾切除正常磷饲料组(STNx+NP);假手术高磷饲料组(Sham+HP);假手术低磷饲料组(Sham+LP);假手术正常磷饲料组(Sham+NP).肾切除组大鼠分两步进行肾脏切除,术前1周及2次手术后7、14、21、28 d在大鼠眼内眦静脉取血,分别检测大鼠血清中血磷(P)、全段甲状旁腺素(iPTH)水平.术后4周取大鼠残余肾脏及甲状腺与甲状旁腺的复合体,进行病理组织学检查.结果:STNx+NP组、STNx+LP组和STNx+HP组术后存活大鼠数分别为5只、4只和4只;假手术各组大鼠未见死亡.肾切除组术后各时段血清iPTH水平均高于术前和各自假手术组(P＜0.05);STNx+HP组血清iPTH水平明显高于STNx+NP组和STNx+LP组(P＜0.05).STNx+HP组术后各时段血清P水平高于术前和Sham+HP组(P＜0.05);而STNx+NP组、STNx+LP组与各自假手术组无显著差异,且均低于STNx+HP组(P＜0.05).STNx+HP组大鼠术后28 d组织病理学观察可见肾小球纤维化及硬化;甲状旁腺组织观察可见腺体增大,腺体中主要见主细胞及甲状旁腺嗜酸性细胞.结论:高磷饮食合并5/6肾切除可建立大鼠慢性肾功能衰竭继发甲状旁腺功能亢进模型,具有动物成本低,操作方法简便,实验周期短,动物死亡率低等优点. |
| 关键词: 肾功能衰竭,慢性、甲状旁腺功能亢进症,继发性、高磷、5/6肾切除、模型,动物 |
| DOI：10.3724/SP.J.1008.2006.00784 |
| 投稿时间：2005-11-25修订日期：2006-05-25 |
| 基金项目:上海市科委重大项目(0452NM035). |
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| High phosphorus foods combined with 5/6 nephrectomy for establishing rat hyperparathyroidism model secondary to chronic renal failure |
| LI Dan,ZHAO Xue-zhi |
| (第二军医大学长征医院肾内科,上海,200003) |
| Abstract: |
| Objective：To establish a rat hyperparathyroidism model secondary to chronic renal failure, so as to lay a foundation for studying the mechanism and treatment of secondary hyperparathyroidism. Methods： Thiry-six male Sprague-Dawley rats, weighing 200-250 g, were randomly divided into 6 groups： 5/6 nephrectomy plus high phosphorus food group （STNx ＋ HP）, 5/6 nephrectomy plus low phosphorus food group （STNx ＋ LP）, 5/6 nephrectomy plus normal phosphorus food group （STNx ＋ NP）, sham operation plus high phosphorus food group（Sham ＋ HP）, sham operation plus low phosphorus food group（Sham ＋ LP）, and sham operation plus normal phosphorus food group（Sham ＋ NP）. Nephrectomy was performed in 2 steps. Serum phosphorus levels （P） and iPTH levels were detected at day 7 pre-operation and day 7, 14, and 21 after the second operation. The kidneys, thyroid glands and parathyroid glands complex underwent pathological analysis 4 weeks after operation. Results： Five patients survived in STNx＋NP group, 4 in STNx＋LP group and 4 in STNx＋HP group postoperatively. No death occurred in sham operated groups. Serum phosphorus levels of nephrectomy groups at different time points after operation were higher than those before operation and those of sham operation groups （P〈 0. 05）. Serum phosphorus level of STNx＋HP group was obviously higher than those before operation and Sham ＋ HP group（P〈0.05）; those of STNx＋NP and STNx＋LP were similar to those of Sham ＋ NP and Sham ＋ LP group, respectively; but were both lower than that of STNx＋HP group（P〈0.05）. Pathological analysis demonstrated renal glomerulus putty denaturalization and sclerosis. Histological examination of parathyroid glands revealed gland hypertrophy, mostly adelomorphous cells and acidophilia cells, in STNx＋ NP group 28 d after operation. Conclusion： Nephrectomy plus high phosphorus foods can be used to establish hyperparathyroidism model secondary to chronic renal failure in rats, with low cost, simple operation, short experiment cycle and low animal mortality. |
| Key words: kidney failure,chronic hyperparathyroidism,secondary high phosphorus 5/6 nephrectomy models,animal |
