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知母中菝葜皂苷元前处理方法探讨
朱东亮,李翔,娄子洋,董昕,柴逸峰
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(第二军医大学药学院药物分析学教研室,上海,200433)
摘要:
目的:对<中国药典>2005版一部中知母药材的菝葜皂苷元含量测定的前处理条件进行改进.方法:采用L9(33)正交设计表,对超声时间、提取溶剂乙醇的比例以及超声次数等因素进行优化.为确保色谱方法的沿用性,还做了加样回收率考察.结果:最终确立了70﹪乙醇超声20 min提取2次作为最优的提取条件,再进行水解得到皂苷元进行含量测定.结论:与药典方法比较,改进后的方法测定的含量结果有了较大提高,能够反映出样品中真实的菝葜皂苷元含量.
关键词:  知母、菝葜皂苷元、提取法
DOI:10.3724/SP.J.1008.2006.00790
投稿时间:2006-04-21修订日期:2006-05-25
基金项目:上海市科委重大项目(2403DZ19548);上海市科委科技攻关项目(02419125).
Pretreatment of sarsasapogenin in Rhizoma Anemarrhenae: a research of methodology
ZHU Dong-liang,LI Xiang,LOU Zi-yang,DONG Xin,CHAI Yi-feng
(第二军医大学药学院药物分析学教研室,上海,200433)
Abstract:
Objective:To improve the pretreatment method described in ChP (2005 Edition, Vol I ) for the determination of sarsasapogenin in Rhizoma Anemarrhenae. Methods: According to orthogonal design L9 (33), the extraction time, alcohol concentration, extraction frequency,etc, were modified to achieve the best extraction outcome with ultrasonic wave extraction. Results: The optimal extraction condition included 70%0 alcohol and twice ultrasonic wave extraction,20 min each time. Finally hydrolization was applied to obtain sarsasapogenin for determination. Conclusion: Compared with the pretreatment method in ChP, this improved extraction method shows a higher sarsasapogenin content in determination and is more accurate in reflecting the true content of sarsasapogenin in Rhizoma Anemarrhene
Key words:  Rhizoma Anemarrhene  sarsasapogenin  extraction