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人眼眶横纹肌肉瘤动物模型的建立
梁莉,魏锐利,马晓晔,蔡季平
0
(武警安徽总队医院眼科,合肥,230041;第二军医大学长征医院眼科,上海,200003)
摘要:
目的:探讨建立人眼眶横纹肌肉瘤动物模型的方法. 方法:培养人胚胎型横纹肌肉瘤细胞株RD细胞,取对数生长期细胞分别接种于4周龄SCID鼠和60Co照射 (1 Gy) 后1 d 的BALB/c裸小鼠;选用成瘤BALB/c裸小鼠的瘤块移植入另一批4周龄BALB/c裸小鼠胁部皮下(组织块接种法).观察各组动物肿瘤生长情况,绘制肿瘤生长曲线;肿瘤组织行常规病理H-E染色并镜检. 结果:60Co照射后1 d裸小鼠成瘤时间约6~8周,SCID鼠组约3~4周,组织块接种法移植2周后裸小鼠瘤体较明显,3组动物的成瘤率均为100﹪;成功绘制出肿瘤生长曲线,肿瘤均呈进行性生长;病理检查示各组移植性横纹肌肉瘤与人体肿瘤标本所见类似.结论:成功建立了裸小鼠和SCID鼠人眼眶横纹肌肉瘤模型,组织块接种法可以缩短BALB/c裸小鼠成瘤时间,为进一步研究人眼眶横纹肌肉瘤奠定了基础.
关键词:  模型,动物、眼眶、横纹肌肉瘤
DOI:10.3724/SP.J.1008.2006.00902
投稿时间:2006-04-17修订日期:2006-06-16
基金项目:上海市科委专项基金(0352nm114).
Establishment of human orbital rhabdomyosarcoma model in mice
LIANG Li,WEI Rui-li,MA Xiao-ye,CAI Ji-ping
(武警安徽总队医院眼科,合肥,230041;第二军医大学长征医院眼科,上海,200003)
Abstract:
Objective:To establish a mouse model of human orbital rhabdomyosarcoma(RD). Methods: RD cells were cultured and implanted subcutaneously into the costal regions of nude mice and severe combined immunodeficiency (SCID) mice (4 weeks old). The nude mice were radiated by ^60Co 1 day before the injection. The rhbdomyosarcoma tissue masses were harvested from nude mice and were subcutaneously implanted into the costal regions of another group of BALB/c mice (tumor tissue mass implantation). The tumor growth was observed in all groups and the growth curves were ploted. H-E staining was used to examine pathology of the tumor tissues. Results: Solid tumors were palpable 6-8 weeks after implantation in ^60Co radiated nude mice and about 3-4 weeks after implantation in SCID mice. The solid tumors were obviously seen in the nude mice 2 weeks after tumor tissue mass transplantation. The tumor forming rates were 100% in all groups. The tumor growing curves were successfully ploted and the tumors had a progressive growth. Pathological findings of the tumor tissue were similar to those of human orbital rhabdomyosarcoma. Conclusion: The human orbital rhabdomyosarcoma models have been successfully established in nude mice and SCID mice. Tumor tissue mass implantation can shorten the tumor forming time in BALB/c nude mice. This research lays a foundation for further study or human orbital rhabdomyosarcoma.
Key words:  models, animal  orbital  rhabdomyosarcoma