| 摘要: |
| 目的:探讨免疫抑制治疗对周围神经损伤再生的影响.方法:49只SD大鼠随机分为7组,每组7只,分别为空白对照组、坐骨神经钳夹、横断、缺损损伤动物模型免疫抑制治疗组以及各自生理盐水对照组.各损伤免疫抑制治疗组大鼠术后腹腔内注射免疫抑制剂环磷酰胺,生理盐水对照组大鼠腹腔内注射生理盐水.术后采用足迹分析、电生理以及组织形态学观察评价神经再生和功能恢复的情况;采用免疫组化方法检测损伤局部自身免疫反应状况.结果:神经损伤后免疫抑制治疗组神经功能指数优于生理盐水对照组,电生理(神经传导速度)和组织形态观察显示免疫抑制治疗组神经功能恢复优于生理盐水对照组.免疫组化结果表明免疫抑制治疗使神经损伤局部免疫球蛋白沉积明显少于生理盐水对照组.结论:免疫抑制治疗可减轻周围神经损伤后发生的自身免疫反应,从而改善了周围神经损伤后再生的微环境,促进了神经的再生. |
| 关键词: 免疫抑制法、环磷酰胺、周围神经、创伤和损伤、神经再生 |
| DOI：10.3724/SP.J.1008.2006.00941 |
| 投稿时间：2006-06-27修订日期：2006-08-28 |
| 基金项目: |
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| Effects of immunosuppression on post-injury regeneration of peripheral nerve in rats |
| SUN Mei-qing,JI Zheng-lun,JIANG Hua,ZHANG Jing-de |
| (第二军医大学长征医院整形外科,上海,200003;第二军医大学长海医院整形外科,上海,200433) |
| Abstract: |
| Objective：To investigate the effects of immunosuppression treatment on peripheral nerve injury and regeneration in rats. Methods; Forty-nine SD rats were randomly divided into 7 groups： sciatic nerve （in the middle of left thigh） forcepscrushing＋ cyclophosphane, transecting ＋ cyclophosphane, resecting groups ＋ cyclophosphane, forceps-crushing ＋ normal saline, transecting＋ normal saline, resecting groups＋ normal saline, and blank control groups. Cyclophosphane and normal saline were intraperitoneally injected into rats post-operatively. Peripheral nerve regeneration and its related function were assessed by walking track analysis,electrophysiology and histomorphology; immunohistochemistry method was used to evaluate the local autoim- mune reactions 12 weeks after operation. Results： Cyclophosphane treated animals had higher scores of sciatic function index （SFI） compared to animals in the corresponding normal saline groups. The electrophysiological （nerve conduction velocity） and morphological examinations showed better regeneration of the myelinated axons in immunosuppression treated animals compared to the corresponding normal saline groups. The immunohistochemistry showed that the intensities of the local immunological response in immunosuppression groups were obviously lower as compared to the corresponding normal saline groups. Conclusion： There is local autoimmune reaction in post traumatic nerve regeneration and this autoimmune reaction may influence nerve regeneration. Cyclophosphane treatment can suppress this autoimmune reaction and improve the micro-environment for nerve regeneration |
| Key words: immunosuppression cyclophosphamide peripheral nerves wounds and injuries nerve regeneration |
