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| 早期高容量血液滤过后多器官功能障碍综合征猪血清IL-6浓度及肺IL-6 mRNA表达的变化及意义 |
| 王冰,方国恩,满晓波,杜成辉 |
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| (解放军南京军区福州总医院普通外科,福州,350025;第二军医大学长海医院普通外科,上海,200433;第二军医大学东方肝胆外科医院细胞信号转导研究中心,上海,200438) |
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| 摘要: |
| 目的:探讨早期高容量血液滤过(HVHF)后多器官功能障碍综合征(MODS)猪血清IL-6浓度及肺组织IL-6 mRNA表达水平的变化及意义.方法:雄性家猪25只随机分为模型组(n=9)、滤过组(n=10)、对照组(n=6).对照组输注生理盐水,模型组和滤过组采用二次打击建立MODS模型,而后滤过组采用HVHF治疗3 d.双夹心ELISA法测定血清IL-6,实时荧光定量RT-PCR技术定量检测肺IL-6 mRNA表达水平.应用Annexin-V/PI双标记流式细胞仪法检测淋巴细胞的凋亡.结果:滤过组滤过后各脏器功能有关指标水平、器官衰竭发生率、动物死亡率较模型组明显降低且有显著差异(P＜0.05);滤过组血清IL-6浓度及肺组织IL-6 mRNA表达水平较之模型组明显降低并相差显著(P＜0.05);滤过组淋巴细胞凋亡率较之模型组降低并相差非常显著(P＜0.01).结论:早期应用HVHF可以降低创伤引起的血清IL-6及肺组织IL-6 mRNA表达升高,并使淋巴细胞凋亡率下降,降低肺衰竭发生率. |
| 关键词: 多器官功能障碍综合征、血液滤过、白细胞介素6、细胞凋亡 |
| DOI：10.3724/SP.J.1008.2006.00961 |
| 投稿时间：2006-05-22修订日期：2006-08-29 |
| 基金项目: |
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| Changes of serum IL-6 levels and pulmonary IL-6 mRNA expression after high-volume hemofiltration in pigs with multiple organ dysfunction syndrome |
| WANG Bing,FANG Guo-en,MAN Xiao-bo,DU Cheng-hui |
| (解放军南京军区福州总医院普通外科,福州,350025;第二军医大学长海医院普通外科,上海,200433;第二军医大学东方肝胆外科医院细胞信号转导研究中心,上海,200438) |
| Abstract: |
| Objeetive..To investigate the effect of high-volume hemofiltration （HVHF） on serum levels of IL-6 and pulmonary expression of IL-6 mRNA in pigs with multiple organ dysfunction syndrome （MODS）. Methods： Twenty-five pigs were randomly divided into model group, control group and HVHF group. Animals in control group were injected with normal saline, and those in the other 2 groups were treated with ＂a second hit＂ to establish MODS model, then animals in HVHF group was treated with HVHF for 3 days. Serum IL-6 levels were determined by ELISA and Real-time quantitative PCR was used to determine the expression level of IL-6mRNA. Lymphocyte apoptosis was detected by Annexin-V/PI flow cytometry. Results： After HVHF, the serum indices associated with organ-function and the morbidity and mortality were much lower than those in MODS model group （P〈0.05）. The expression level of IL-6 mRNA in lung was reduced significantly in HVHF group com- pared with that in model group （P〈0.05）; moreover, the lymphocyte apoptosis rate was also significantly decreased compared with model group （P〈0.01）. Conclusion.. Early HVHF can decrease the high serum levels of IL-6 and the over-expression of IL-6 mRNA in lungs caused by trauma; it can also decrease the lymphocyte apoptosis rate, so as to prevent the incidence of lung failure |
| Key words: multiple organ dysfanctious syndrome hemofiltration interleukin-6 apoptosis |
