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丹参总酚酸抑制大鼠肾间质纤维化的初步观察
尚雁君,黄才国,蒋山好,朱大元,魏善建,焦炳华,李锴男,张建荣
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(第二军医大学基础医学部生物化学和分子生物学教研室,,200433)
摘要:
目的:观察丹参总酚酸对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响。方法:将 40只健康、雄性 SD大鼠随机分为4组:分别为UUO组、假手术组(SOR)、阳性对照依那普利(ACEI)组和丹参总酚酸样品(TSA)组,术前分别给UUO组和SOR组灌生理盐水,ACEI组和丹参总酚酸组分别灌胃ACEI\[6 mg/(kg·d)\]和丹参总酚酸\[20 mg/(kg·d)\],连续8 d。术后第 7天分别处死各组大鼠用免疫组织化学方法测定 TGF-β1的表达情况。行H-E和 Masson染色,动态观察肾间质病理学改变。结果:丹参总酚酸组和ACEI组TGF-β1的表达量与模型组(0.22±0.06)比较有明显的下降(P<0.05), 结果分别是1.38±0.26和1.38±0.26。丹参总酚酸能显著减少UUO大鼠肾小管间质TGF-β1的表达,减轻胶原在肾间质的沉积,改善了肾脏病理改变。结论:丹参总酚酸对UUO所致的大鼠肾脏纤维化的形成有明显的抑制作用,其作用可能跟降低TGF-β1的表达有关。
关键词:  丹参总酚酸  肾间质纤维化  转化生长因子β1?
DOI:10.3724/SP.J.1008.2006.01295
基金项目:
Total salvianolic acid inhibiting renal interstitial fibrosis in rats?
()
Abstract:
Objective:To study the influence of total salvianolic acid(TSA) on the renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats. Methods: Forty healthy Sprague-Dawley rats were randomly divided into 4 groups:the UUO group, sham-operation group, angiotensin-converting enzyme inhibitor (ACEI)-treated group (positive control), and TSA-treated group. The rats in UUO group and sham-operation group received gastric gavage with normal saline for 8 days before operation; rats in ACEI-treated group and TSA-treated group received ACEI (6 mg/kg by daily gastric gavage for 8 days) and TSA (20 mg/kg by daily gastric gavage for 8 days), respectively. All rats were killed 7 days after operation and the expression of transforming growth factor-β1 (TGF-β1) was detected by immunohistochemical method. The dynamic histological changes of renal interstitial tissues were observed by H-E and Masson staining.Results: TGF-β1 expression in TSA-treated (1.38±0.26) and ACEI-treated (1.38±0.26) groups was significantly lower than that in UUO group(P<0.05). TSA obviously reduced TGF-β1 expression and collagen deposition in the renal interstitial tissues and improved the renal pathological changes in UUO rats. Conclusion: TSA can evidently inhibit UUO-induced renal interstitial fibrosis in rats, which might be related with the down regulation of TGF-β1 expression.
Key words:  total salvianolic acid  renal interstitial fibrosis  transforming growth factor-β1 ?