| 摘要: |
| 目的：观察低氧诱导因子1α（hypoxia inducible factor-α，HIF-1α）在低氧培养的肝癌细胞中的表达变化，并初步探讨其与血管生成和细胞凋亡的关系。方法：将终浓度为125μmol／L的氯化钴加入HepG2肝癌细胞培养液中模拟低氧环境，并设培养不同时间（O、1、2、4、6、8h）组。（1）采用RT-PCR技术检测低氧培养不同时间的肝癌细胞中HIF-1α、VEGF、Caspase-3、bcl-2和bax mRNA的表达；（2）应用Western印迹方法及酶标免疫测量仪分别检测了各培养组细胞Caspase-3蛋白表达及Caspase-3酶活性。结果：（1）HepG2细胞在低氧培养1～2h后，其HIF-1n、VEGF、bob2mRNA的表达逐渐增加，在4h达到高峰，随后下降，但仍高于低氧处理前水平；而baxmRNA表达无明显变化，bcl-2／bax比值与上述变化趋势一致。（2）Caspase-3mRNA及蛋白的表达及酶活性变化趋势与HIF-1α正好相反。结论：HI卜1n可能通过调节VEGF、bcl-2、bax和Caspase-3的表达而抑制肝癌细胞凋亡，且这种抑制作用与缺氧程度有关。[著者文摘] |
| 关键词: 低氧诱导因子 血管内皮生长因子类 基因 bcl-2 半胱氨酸天冬氨酸蛋白酶 肝肿瘤 |
| DOI：10.3724/SP.J.1008.2006.01328 |
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| 基金项目: |
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| Expression of hypoxia inducible factor-1α in HepG2 cells cultured under hypoxia and its relationship with vascularization and apoptosisorelated protein |
| FU Zhi-chao , WANG Feng-mei, CHENG Hui-hua |
| (1. Department of Radiotherapy, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025, China;2. Department of Obstetrics and Gynecology, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350025) |
| Abstract: |
| Objective：To investigate the expression of hypoxia inducible factor-1α（HIF-1α） in HepG2 cells cultured under hypoxia for different time periods, and to assess its relationship with vascularization and cell apoptosis. Methods： HepG2 cells were cultured in a medium containing cobalt chloride （125μmol/L） for different time periods （ 0, 1, 2, 4, 6, and 8 hours ）. RT-PCR was used to measure the expression of HIF-1α mRNA,VEGF mRNA,and bcl-2 mRNA, and bax mRNA at the above time points; Western blot was used to determine the expression of Caspase-3 protein and the activity of Caspase-3 enzyme. Results： After 1-2 hour culture under hypoxia, the expression of HIF-1α mRNA, VEGF mRNA,and bcl-2 mRNA began to increase gradually, peaked at 4 h, and then decreased, but were still higher than that before culture. There was no obvious change in the expression of bax mRNA and the ratio of bcl-2 to bax mRNA expression had a similar change with that of bcl-2 mRNA expression. The changes of Caspase-3 protein/mRNA and Caspase-3 enzyme activity were contrary to that of HIF-1α. Conclusion： HIF-1α may inhibit the apoptosis of hepatic cancer ceils through regulating the expression of VEGF, hcl-2, bax, and Caspase-3, and the inhibition is related to the severity of hypoxia.[著者文摘] |
| Key words: hypoxia inducible factor-1α vascular endothelial growth factors genes,hcl-2 Caspases liver neoplasmas |
