| 摘要: |
| 目的：从Th1/Th2平衡角度出发探讨多器官功能障碍综合征（MODS）的发病机制。方法：将体质量22～30 kg健康雄性家猪随机分为MODS组和正常对照组，每组各9只。采用失血性休克复苏+内毒素血症复合因素建立猪MODS模型。MODS组于放血前，内毒素注射前，内毒素注射后1、24、48及72 h留取血标本，动物死亡后留取脾脏标本。正常对照组同时间点留取标本。ELISA法检测血清IFN-γ和IL-4含量变化；实时荧光PCR法测定脾脏IFN-γ、IL-4 mRNA水平及Th1/Th2细胞特异性转录因子T-bet和GATA-3 mRNA的含量变化。结果：内毒素注射后1 h血清IFN-γ和IL-4浓度达到峰值，后IFN-γ浓度下降较快，内毒素注射后72 h IFN-γ/IL-4比值显著低于基础值水平（P=0.000）；MODS组脾脏IFN-γ/IL-4 mRNA比值降低明显，与对照组比较差异具有显著性（P=0.020）；脾脏T-bet/GATA-3比值也显著低于对照组（P=0.038）。结论：MODS发生过程中存在Th1向Th2漂移现象。 |
| 关键词: 多器官功能衰竭 干扰素Ⅱ型 白细胞介素4 转录因子 |
| DOI：10.3724/SP.J.1008.2007.01347 |
| 投稿时间：2007-01-24 |
| 基金项目:军队“十五”医学研究重点课题(01L006). |
|
| Changes of Th1/Th2 balance in multiple organ dysfunction syndrome |
| SHEN Xian, CHAI Chen, LIN Fu-lin, SHI Jun-yi, FANG Guo-en* |
| (Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective：To explore the pathogenesis of multiple organ dysfunction syndrome (MODS) with respect to the balance of Th1/Th2. Methods: Eighteen healthy male minipigs, weighing 22-30 kg, were randomly divided into two groups: MODS group and control group. Double-hit method including hemorrhagic shock and endotoxiemia was used to establish the porcine MODS model.The peripheral vein blood samples were collected at different time-points (before bloodletting, before endotoxin injection, 1 h, 24 h,48 h and 72 h after endotoxin injection) in the two groups. The spleen samples were collected after death of the animals. Plasma levels of IFN-γ and IL-4 were detected by enzyme-linked immunosorbent assay (ELISA). Real-time PCR was used to detect the expression of IFN-γ, IL-4, T-bet and GATA-3 mRNA (The latter 2 were the key transcription factors associated with Th1/Th2 response) in the spleen samples.Results: The plasma levels of IFN-γ and IL-4 quickly reached the peak values 1 h after the endotoxin injection, then the level of IFN-γ decreased quickly. The ratio of IFN-γ/IL-4 was significantly lower than the baseline value 72 h after endotoxin injection（P=0.000）. The ratio of IFN-γ/IL-4 mRNA in MODS group was obviously lower than that in the control group（P=0.020）; the ratio of T-bet/GATA-3 was also lower in MODS group（P=0.038）.Conclusion: The shift from Th1 to Th2 occurs in the progress of MODS. |
| Key words: multiple organ failure interferon typeⅡ interleukin-4 transcription factors |
