| 摘要: |
| 目的：探讨瘦素(leptin)对于大鼠肠缺血再灌注损伤的保护作用及其机制。方法：建立大鼠肠缺血再灌注损伤模型，99只Wistar大鼠随机分成11组：假手术组(sham)、肠缺血45 min再灌注15 min组(I45R15)、I45R30、I45R60、I45R180、I45R360、和相应时间点的leptin(0.2 mg/kg)治疗组，每组9只。检测再灌注不同时间点大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素10(IL-10)水平的动态变化，并观察小肠组织病理学的变化。结果：与假手术组相比，肠缺血再灌注组血清中TNF-α随着时间变化显著升高，IL-10水平也高于假手术组(P＜0.05)，病理显示肠道明显损伤；与肠缺血再灌注组相比，Leptin治疗后血清中的TNF-α水平显著降低，IL-10水平显著升高，小肠组织损伤明显减轻(P＜0.05)。结论：Leptin可通过下调血清中TNF-α、上调IL-10水平减轻肠缺血再灌注损伤对大鼠肠道的局部损伤。 |
| 关键词: 再灌注损伤 瘦素 肠黏膜 细胞因子 |
| DOI：10.3724/SP.J.1008.2008.00250 |
| 投稿时间：2007-02-06修订日期：2008-01-26 |
| 基金项目:国家自然科学基金面上项目(青年科学基金项目，30600563). |
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| Effect of leptin on serum TNF-α and IL-10 levels and small intestine damage in rats with intestine ischemia/reperfusion injury |
| WANG Xiao-hui,WANG Lu-huan,XUE Hui,YAN Guang-tao*,DENG Zi-hui |
| (Laboratory of Biochemistry,Basic Medical Institute,General Hospital of PLA,Beijing 100853,China) |
| Abstract: |
| Objective：To explore the protective effect of leptin against rats intestinal ischemia/reperfusion injury and the related mechanism. Methods: Ninety-nine rats were randomly divided into 11 groups (n=9),namely the sham-operation group,ischemia/reperfusion groups (45 min ischemia plus 15 reperfusion \[I45R15\],I45R30,I45R60,I45R180,I45R360)and the 5 corresponding leptin-treated ischemia-reperfusion groups. The serum levels of TNF-α and IL-10 were determined in all groups and the histopathological changes were observed in the small intestine. Results: Compared with the sham-operation group,the serum levels of TNF-α and IL-10 were significantly increased in the ischemia/reperfusion groups (both P<0.05),with obvious pathological damage observed in the ischemia/reperfusion groups. Leptin treatment significantly decreased TNF-α level,increased IL-10 level,and alleviated the damage of the small intestine (all P<0.05) of animals after ischemia /reperfusion injury.Conclusion: Leptin can alleviate the intestinal damage in rats after intestinal ischemia/reperfusion through down-regulating TNF-α level and up-regulating IL-10 levels. |
| Key words: reperfusion injury leptin intestinal mucosa cytokine |
