摘要: |
目的:通过对人胸腺素α原(prothymosin-α, ProTα)cDNA测序来分析人胸腺素α原的序列多态性。方法:应用RT-PCR技术从健康人外周血及健康新生儿脐带血中扩增胸腺素α原cDNA,纯化后与克隆载体pMD18-T连接,进行克隆测序,并与标准序列比对,分析其多态性。 结果:序列分析结果表明,克隆的ProTα基因的核苷酸序列并不一致。与已报道的胸腺素α 原基因(NM-002823)进行比较,发现存在2种变异:Ⅰ类变异包括107位单核苷酸突变(A→G)、110~121位和191~205位的核苷酸片段缺失;Ⅱ类变异为306位单核苷酸(G)缺失,多见于年龄60~80岁者。 结论:本研究中ProTα cDNA序列存在2种变异,但并未影响其N-端前28个氨基酸。 |
关键词: 胸腺素α原 cDNA 序列分析 |
DOI:10.3724/SP.J.1008.2008.00092 |
投稿时间:2007-02-12 |
基金项目: |
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Analysis of polymorphism in human cDNA sequence of prothymosin-α |
GONG Xue-lian, GUO Bao-yu*, GUO Man-ying, Lv Yan |
(Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
Abstract: |
Objective:To analyze the polymorphism in human cDNA sequence of prothymosin-α (ProTα) by sequencing analysis. Methods: The cDNA of human ProTα was amplified from cells of peripheral blood and cord blood by RT-PCR.The product of RT-PCR was purified and linked with vector pMD18-T. After cloning and sequencing, the sequence of ProTα cDNA was compared with the standard sequence to analyze the polymorphism in the ProTα cDNA sequence. Results: The cloned ProTα cDNA sequence was different from that of the standard. We found 2 kinds of variations: (1) The nucleotide in 107 position was varied and the nucleotides in 110-121 and 191-205 positions were deleted; (2) The nucleotide in 306 position was deleted, mainly in the 60-80 years old group. Conclusion: We have identified 2 kinds of variations in human ProTα cDNA, but the first 28 amino acid in the N-terminal of cDNA of human ProTα are not involved therefore the variations do not affect the function of human ProTα. |
Key words: prothymosin-α cDNA sequence analysis |