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HPLC-ELSD法测定青葙子中青葙苷A和青葙苷B的含量
王莹1,2,郭美丽1*,王笑康2,殷军2
0
(1. 第二军医大学药学院生药学教研室,上海 200433;2. 沈阳药科大学中药学院生药学教研室,沈阳 110016)
摘要:
目的:测定青葙子中青葙苷A和青葙苷B的含量。 方法:HPLCELSD法,色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈0.1%冰乙酸水溶液梯度洗脱,以蒸发光散射检测器(ELSD)检测。 结果:青葙苷A在2.5~30.0 μg范围内与峰面积呈良好的线性关系,回收率为99.89%,RSD为1.85% (n=6);青葙苷B在1.25~15.0 μg范围内与峰面积呈良好的线性关系,回收率为98.98%,RSD为1.78% (n=6)。8个市售青葙子样品中青葙苷A和青葙苷B的含量存在显著差异,分别在(0.051 2±0.001 6)%~(0.114 3±0.000 8)%和(0.010 9±0.001 9)%~(0.081 5±0.000 9)%范围内。 结论:本研究首次建立了用HPLCELSD法测定青葙子有效成分的方法,该方法定量准确、操作简便。
关键词:  青葙子  青葙苷A  青葙苷B  色谱法,高压液相  蒸发光散射检测器
DOI:10.3724/SP.J.1008.2007.01245
投稿时间:2007-04-30
基金项目:上海市中药现代化专项基金(06DE19715);军队“十一五”中医药研发推广专项基金(2006172003).
HPLC-ELSD in determination of celosin A and celosin B contents in Semen celosiae
WANG Ying1,2,GUO Mei-li1*, WANG Xiao-kang2, YIN Jun2
(1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2. Department of Pharmacognosy, College of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016)
Abstract:
Objective:To determine the contents of celosin A and celosin B in Semen celosiae.Methods: HPLC was carried out with an Agilent Alliance, Model 1100, equipped with Elite ODS C18 column (4.6 mm×250 mm,5 μm) and evaporated light scattering detector (ELSD). The mobile phase (acetonitrile0.1% glacial acetic acid) was eluted in gradient mode.Results: Celosin A and the peak area showed a good linearship within the range of 2.530.0 μg, with the recovery rate being 99.89% (n=6) and RSD being 1.85%. Celonsin B and the peak area showed a good linearship within the range of 1.2515.0 μg, with the recovery rate being 98.98% (n=6) and the RSD being 1.78%. Contents of celosin A and celosin B in 8 Semen celosiae samples on medical markets were obviously different from each other, ranging from(0.051 2±0.001 6)% to (0.114 3±0.000 8)% and (0.010 9±0.001 9)% to (0.081 5±0.000 9)%.Conclusion: We have established an HPLCELSD method for determining the effective contents of Semen celosiae; the method is accurate and simple.
Key words:  Semen celosiae  celosin A  celosin B  chromatography,high pressure liquid  evaporative light scattering detector