| 摘要: |
| 目的：观察华中五味子酮（Schisandrone）对阿尔茨海默病（Alzheimer’s disease，AD）样大鼠学习记忆及海马区核因子κB（nuclear factor-κB）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）表达的影响，探讨华中五味子酮对AD可能的防治作用。方法：30只雄性SD大鼠随机分为空白对照组、AD模型组和华中五味子酮干预组3组，每组各10只。采用侧脑室立体定向注射β淀粉样蛋白(amyloid-beta protein, Aβ)25-35的方法，建立AD的动物模型；华中五味子酮干预组采用华中五味子酮灌胃进行药物干预，空白对照组注射生理盐水。通过Morris水迷宫检测大鼠学习、记忆能力，通过免疫组化法观察大鼠海马区NF-κB及iNOS 蛋白的表达。结果：华中五味子酮干预组大鼠短期学习记忆能力较AD样大鼠有明显改善（P<0. 05），海马区NF-κB及iNOS 的表达较AD样大鼠明显减少（P<0. 05），海马区NF-κB及iNOS 的表达呈正相关关系（空白对照组、AD模型组、华中五味子酮干预组的相关系数分别为0.639、0.656、0.682,P均<0. 05）。结论：华中五味子酮可能通过影响NF-κB信号转导通路而抑制Aβ诱导的氧化应激和炎性反应，在AD发病中具有保护作用。 |
| 关键词: 阿尔茨海默病 核因子κB 诱导型一氧化氮合酶 华中五味子酮 迷宫学习 |
| DOI：10.3724/SP.J.1008.2007.01351 |
| 投稿时间：2007-05-11 |
| 基金项目: |
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| Schisandrone improves learning and memory abilities of Alzheimer-like rats and influences expression of NF-κB, iNOS in rat hippocampus |
| YU Fang1，TUO Xi-ping1*，L Jian-yong1，CHEN Hai-sheng2 |
| (1.Department of Geriatrics，Changhai Hospital，Second Military Medical University，Shanghai 200433，China;2.Department of Pharmacochemistry, School of Pharmacy, Second Military Medical University，Shanghai 200438) |
| Abstract: |
| Objective：To investigate the influence of Schisandrone on the learning and memory abilities of rats with Alzheimer-like disease and on the expression of NF-κB, iNOS in rat hippocampus, so as to study the prevention effect of Schisandrone on Alzheimer disease (AD). Methods: Totally 30 male SD rats were evenly randomized into 3 groups: blank control group, AD model group and Schisandrone intervention group. The AD animal model was established by stereotactic injection of Aβ25-35 into lateral cerebral ventricle of rats; the rats in Schisandrone intervention group were administrated with Schisandrone. The learning and memory abilities of animals were determined by Morris water maze; the expression of NF-κB, iNOS in the hippocampus was detected by immunohistochemistry. Results: The learning and memory abilities of rats in the Schisandrone intervention group were significantly improved compared with those in the AD model group (P＜0.05). The expression of NF-κB and iNOS in the hippocampus was significantly decreased in the Schisandrone group than in the AD model group（P<0.05）. The expression of NF-κB and iNOS in the hippocampus was positively correlated with each other. The correlation coefficients for the blank control,AD model and Schisandrone intervention groups were 0.639,0.656 and 0.682,respectively （all P<0. 05）. Conclusion: Schisandrone can suppress the Aβ-induced oxidative stress and inflammatory reaction through influencing NF-κB signaling pathway, exerting its protective effect on AD. |
| Key words: Alzheimer disease nuclear factor-kappa B inducible nitric oxide synthase schisandrone maze learning |
