| 摘要: |
| 目的：研究骨形成蛋白（bone morphogenetic protein，BMP）-2在犬面中份缝牵引成骨过程中的表达，探讨缝牵引成骨的机制。方法：18只12周龄杂种犬随机分为牵引成骨组12只和正常对照组6只,在牵引5、10、15 d，固定10、20 d，去固定1个月分别处死实验犬2只和对照犬1只，免疫组化法观察BMP-2在各骨缝的表达情况。结果：牵引期和固定期，BMP-2在实验犬各骨缝边缘大量增殖的未成熟成骨细胞、间充质细胞以及新生骨基质中呈阳性表达，对照犬也有少量表达。随着机械应力牵引, BMP-2在各骨缝表达量逐渐上升，在牵引15 d阳性表达最强，以后逐渐下降，到去固定1个月时表达水平接近正常。结论：机械牵张力刺激可以导致内源性BMP-2产生，BMP-2可能在缝牵引新骨生成过程中扮演重要的角色。 |
| 关键词: 骨形成蛋白2 面中份 缝牵引成骨术 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2007.01356 |
| 投稿时间：2007-05-25 |
| 基金项目:北京市自然科学基金(7072075). |
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| Expression of bone morphogenetic protein-2 during midfacial sutural distraction osteogenesis in dogs |
| JIN Zeng-qiang 1，LIU Chang-fa2，MA Xiao 1，LIU Chun-ming1* |
| (1.Department of Stomatology, General Hospital of PLA，Beijing 100853, China; 2. Zhengzhou Railway Occupational and Technological Institute, Zhengzhou 450052) |
| Abstract: |
| Objective：To study the expression of bone morphogenetic protein (BMP)-2 during midfacial sutural distraction osteogenesis in dogs and to probe the mechanism of sutural distraction osteogenesis. Methods: Eighteen mongrel dogs (12-week old) were randomly divided into distraction osteogenesis group(n=12) and the control group(n=6).Rigid external distractor was used for sutural distraction of the midfacial skeleton in the experimental animals. Two experimental and one control dogs were sacrificed at defined time points (on the 5th, 10th and 15th day of distraction, the 10th and 20th day of fixation, and 30th day after completion of fixation).The expression of BMP-2 was observed by means of immunohistochemistry. Results: During the period of distraction and fixation, positive staining of BMP-2 was found in the proliferated preosteoblasts, the mesenchymal cells and the newly formed bone matrix at the sutural margin of the experimental dogs; weak expression was also noticed in the control dogs. Compared with that in the control group, the expression of BMP-2 in the experimental group increased and reached the peak on the 15th of distraction, then gradually decreased. the expression of BMP-2 nearly restored to the normal level 1 month after the removal of the distractor. Conclusion: The mechanical strain can lead to the generation of endogenous BMP-2, which may play an important role in the new bone formation. |
| Key words: bone morphogenetic protein-2 midface suture distraction osteogenesis immunohistochemistry |
