| 摘要: |
| 目的：观察重复经颅磁刺激(rTMS)对帕金森病(PD)小鼠黑质多巴胺能神经元及脑源性神经营养因子(BDNF)表达的影响，探讨其可能的作用机制。方法：32只雄性C57BL/6J小鼠随机分为生理盐水(NS)、PD模型(PD)、假刺激(s-rTMS)及磁刺激(rTMS)组，每组8只。后3组采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)皮下注射建立PD小鼠模型。rTMS组鼠每天接受1 Hz、1 T 的rTMS治疗(共5个序列，25脉冲/序列)，疗程为2周。经rTMS干预后，免疫组织化学检测黑质(SN)区酪氨酸羟化酶(TH)和BDNF的表达变化，并借助图像分析系统进行定量分析。结果：PD组酪氨酸羟化酶免疫组化阳性(TH-ir)和BDNF免疫组化阳性(BDNF-ir)细胞计数、校正光密度值(CD)较NS组减少(P<0.01)；rTMS组TH-ir和BDNF-ir、CD值较PD组和s-rTMS组增加(P<0.05)；s-rTMS组与PD组间以上指标无统计学差异。相关分析显示黑质区TH-ir与BDNF-ir细胞计数呈正相关(r=0.949,P<0.01)，相应的CD值比较亦呈正相关(r=0.880,P<0.01)。结论：rTMS对PD小鼠模型黑质多巴胺能神经元具有保护作用，而上调黑质区BDNF的表达可能是其作用机制之一。 |
| 关键词: 磁刺激 帕金森病 黑质 酪氨酸羟化酶 脑源性神经营养因子 |
| DOI：10.3724/SP.J.1008.2008.00245 |
| 投稿时间：2007-09-13修订日期：2007-11-23 |
| 基金项目:河北省自然科学基金(C2007000842). |
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| Effect of transcranial magnetic stimulation on dopaminergic neurons and expression of brain-derived neurotrophic factor in substantia nigra of mouse with Parkinson's disease |
| DONG Qiao-yun2,GU Ping1,2,WANG Quan-dong2,CUI Dong-sheng2,WANG Yan-yong1,ZHANG Zhen-qing1,GENG Yuan2,GUO Ji-hong1,WANG Ming-wei1,2* |
| (2. Hebei Key Laboratory for Brain Aging and Congnitive Neuroscience, Shijiazhuang 050031;1. Department of Neurology, First Affiliated Hospital of Hebei Medical University, Shijiazhuang 050031, China) |
| Abstract: |
| Objective:To investigate the effect of repetitive transcranial magnetic stimulation(rTMS)on dopaminergic neurons and brain-derived neurotrophic factor(BDNF) in the substantia nigra (SN) of mouse with Parkinson’s disease (PD ), and to disclose the possible mechanisms. Methods: Thirty-two male C57BL/6J mice were equally randomized into normal saline (NS), sham-rTMS (s-rTMS), PD model and rTMS groups. PD model was established with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice. The animals in rTMS group received 5 trains of 1 pulse/s for 25 s, at an intensity of 1 Tesla (T) daily for 2 weeks. After the treatment of rTMS, the changes in expression of tyrosinehy droxylase (TH) and BDNF in the SN of animals were observed by immunohistochemical technique; the quantitative analysis was performed by image analysis system. Results: Compared with NS group, the numbers of TH and BDNF immunoreactive(TH-ir and BDNF-ir)cells and the corrected optical density (CD) values in PD and s-rTMS group were significantly lower than those in the NS group (all P<0.01); the numbers in rTMS group were significantly higher compared with those in the PD and s-rTMS groups (all P<0.05); and the numbers were not significantly different between the s-rTMS and PD groups(P>0.05). Correlation analysis showed a positive correlation between the count of TH-ir and BDNF-ir cells (r=0.949, P<0.01); and positive correlation was also noted between the CD values of TH-ir and BDNF-ir cells (r=0.880, P<0.01).Conclusion: It is suggested that rTMS has protective effects on dopaminergic neurons in the SN of PD mice, and the mechanism might be related to the up-regulation of BDNF expression. |
| Key words: magnetic stimulation Parkinson disease substantia nigra tyrosine hydroxylase brain-derived neurotrophic factor |
