| 摘要: |
| 目的：观察骨桥蛋白（osteopontin,OPN）在骨巨细胞瘤肿瘤组织中的表达并探讨其可能的临床意义。方法：EnVisionTM二步法检测77例骨巨细胞瘤患者肿瘤组织中OPN的表达，比较不同临床、病理指标骨巨细胞瘤患者间OPN的表达差异，分析其可能的临床意义。结果：OPN在骨巨细胞瘤中表达的阳性率为76.6%，而在正常骨组织中呈弱阳性表达。侵袭性骨巨细胞瘤OPN阳性表达率明显高于非侵袭性骨巨细胞瘤，差别具有统计学意义（95.5% vs 69.1%, P=0.013）。OPN在不同Campanacci分级的肿瘤组织中均有阳性表达，且随着分级的增加，阳性表达率增加，不同分级间阳性表达率差别有统计学意义（P=0.019）。OPN阳性表达率与复发与否、性别、年龄、手术方式、病程长短、发病部位等临床特征无显著相关。结论：骨巨细胞瘤OPN高表达，且与肿瘤侵袭性及分级密切相关。 |
| 关键词: 骨桥蛋白 骨巨细胞瘤 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2008.00423 |
| 投稿时间：2007-10-21修订日期：2008-01-11 |
| 基金项目: |
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| Expression of osteopontin in giant cell tumor of bone and its clinical significance |
| ZHAO Liang-yu1,LIU Yan1,CHEN Ai-min1,GOU San-huai1,HE Jin2,XU Li-ying1,YUAN Wen1* |
| (1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Department of orthopedics, Changzheng Hospital, Second Military Medical University;2. Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003) |
| Abstract: |
| Objective：To observe the expression of osteopontin (OPN) in the giant cell tumor of bone (GCTB) and to discuss its clinical significance.Methods: OPN expression was examined in 77 GCTB samples by EnVisionTM two-step immunohistochemical method. And the expression was compared between patients with different clinical and pathological data; the implied clinical relevance was also analyzed. Results: The positive expression rate of OPN in the GCTB was 76.6%; only weak expression was noted in the normal bone tissues. The expression in the invasive GCTB was significantly higher than that in the non-invasive ones（95.5% vs 69.1%, P=0.013）. The expression rate of OPN increased with the increase of Campanacci stages of GCTB, and significant difference was found between different stages（P=0.019）. The recurrence status, gender, age, surgery types, duration of illness or site of tumor were not associated with the positive rate of OPN. Conclusion: OPN is highly expressed in GCTB tissues, and the expression is associated with the invasiveness and the Campanacci stages of GCTB. |
| Key words: osteopontin giant cell tumor of bone immunohistochemistry |
