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| PSA特异性树突状细胞瘤苗过继免疫治疗小鼠前列腺癌 |
| 徐丹枫*,高轶,刘玉杉*,崔心刚,车建平,阴雷,邢继章,姚亚成,任吉忠,闵志廉 |
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| (第二军医大学长征医院泌尿外科,全军泌尿外科中心,上海 200003) |
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| 摘要: |
| 目的:利用LNCaP前列腺癌裸鼠模型,观察PSA特异性树突状细胞(dendritic cell,DC)瘤苗(PSA-DC)体外诱导的CTL体内过继输入的抗肿瘤效果。方法:采用裸鼠皮下接种LNCaP细胞的方法建立LNCaP前列腺癌荷瘤裸鼠模型;应用前期制备好的Non-DC、Ova-DC、Lys-DC及PSA-DC瘤苗体外诱导抗原特异性CTL细胞,并在LNCaP细胞接种第15天模型制备成功时首次将体外诱导的抗原特异性CTL细胞经尾静脉过继输入小鼠体内,7 d后重复输入1次。以初次治疗后50 d为终止点,观察肿瘤生长情况;观察各组荷瘤裸鼠的存活情况。结果:过继输入治疗后第30、50天,Non-DC、Ova-DC、Lys-DC及PSA-DC组瘤体明显大于肿瘤细胞接种第15天(P<0.01);Lys-DC、PSA-DC组瘤体明显小于Non-DC、Ova-DC组(P<0.01)。在观察期间内,Lys-DC、PSA-DC组裸鼠生存时间明显长于Non-DC、Ova-DC组(P<0.01)。结论:前列腺癌瘤苗PSA-DC体外诱导的PSA特异性CTL细胞过继输入可抑制裸鼠体内LNCaP肿瘤生长,提高裸鼠生存时间。 |
| 关键词: PSA 树突状细胞 细胞毒性T淋巴细胞 过继免疫 前列腺肿瘤 |
| DOI：10.3724/SP.J.1008.2009.0521 |
| 投稿时间：2007-12-14修订日期：2009-03-18 |
| 基金项目:上海市科学技术委员会基金(044119615). |
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| Adoptive immunotherapy with CTL induced by PSA-pulsed dendritic cell vaccine for prostate cancer in mice |
| XU Dan-feng*,GAO Yi,LIU Yu-shan*,CUI Xin-gang,CHE Jian-ping,YIN Lei,XING Ji-zhang,YAO Ya-cheng,REN Ji-zhong,MIN Zhi-lian |
| (Department of Urology,Changzheng Hospital,Second Military Medical University,Center of Urology of PLA,Shanghai 200003,China) |
| Abstract: |
| Objective:To observe the anti-tumor effect of adoptive immunotherapy with CTL induced by PSA-pulsed dendritic cell vaccine against prostate cancer in mice.Methods: Nude mice were s.c.inoculated with LNCaP tumor cells to establish prostate cancer model.CTL induced in vitro by different peptide-pulsed dendritic cells (Non-DC,Ova-DC,Lys-DC and PSA-DC) were used for adoptive immunotherapy of experiment nude model through vena caudalis on d 15 and d 22 after inoculation.The survival of nude mice and the sizes of tumors were observed with d 50 after the initial treatment as the endpoint.Results: The tumor sizes in Non-DC,Ova-DC,Lys-DC and PSA-DC groups on d 30 and d 50 after adoptive immunotherapy were significantly larger than that at d 15 after inoculation (P<0.01).The tumors sizes in Lys-DC and PSA-DC groups were significantly smaller than those in the Non-DC and Ova-DC groups（P<0.01）.The survival periods of animals in Lys-DC and PSA-DC groups were significantly longer than those in the Non-DC and Ova-DC groups (P<0.01）.Conclusion: Adoptive immunotherapy with CTL induced by DC-based PSA vaccine can inhibit the growth of prostate cancer LNCaP cells in nude mice and increase the survival of the animals. |
| Key words: PSA dendritic cell cytotoxic T-lymphocytes adoptive immunity prostatic neoplasms |
