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尼古丁给药和撤药模型的制备与评价
蓝晓红,徐添颖,缪朝玉*
0
(第二军医大学药学院药理学教研室,上海 200433)
摘要:
目的:制备一种尼古丁给药和撤药大鼠模型,通过三项相关指标评价该模型的特征与应用研究价值。方法:10~11周龄的雄性SD大鼠随机分为生理盐水组、尼古丁给药组和尼古丁撤药组3组。生理盐水组给生理盐水6周;尼古丁给药组给尼古丁(3 mg·kg-1·d-1)6周;尼古丁撤药组先给尼古丁(3 mg·kg-1·d-1)3周,随后改为给生理盐水3周。各组给药途径均为皮下注射,每日1次,给药容积为0.5 ml/kg,同时记录体质量、饮食量和饮水量的变化。造模后通过体质量、血清生化指标、各部位脂肪称量来评价该模型。结果:尼古丁给药后体质量下降;血清三酰甘油、胰岛素水平降低;皮下脂肪、内脏脂肪和主动脉周围脂肪质量减少;撤药后上述指标回升。结论:该模型可用于研究尼古丁的多种药理作用,以及吸烟、戒烟对机体的影响。
关键词:  尼古丁  吸烟  吸烟戒断  动物模型  脂肪类  体质量  主动脉
DOI:10.3724/SP.J.1008.2008.00156
投稿时间:2007-12-24修订日期:2008-01-23
基金项目:国家杰出青年科学基金(30525045);全国优秀博士学位论文作者专项资金(200369);上海市科学技术委员会基金(上海市浦江人才计划, 05PJ14002).
Establishment and evaluation of a nicotine treatment and withdrawal rat model
LAN Xiao-hong,XU Tian-ying,MIAO Chao-yu*
(Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China)
Abstract:
Objective:To establish a nicotinetreatment and withdrawal rat model and to evaluate its characteristics and application through analyzing 3 parameters. Methods:Male SpragueDawley rats, aged 1011 weeks old, were randomly divided into normal saline group(subcutaneous injection of saline \[0.5 ml/kg\] for 6 weeks), nicotinetreated group (injection with nicotine \[0.5 ml/kg, 3 mg/(kg·d)\] for 6 weeks), and nicotinewithdrawn group (injection with nicotine \[0.5 ml/kg, 3 mg/(kg·d)\] for 3 weeks and followed by saline injection for additional 3 weeks). Body weight, food intake, and water intake of animals were recorded during the treatment in 3 groups. The model was evaluated through analyzing body weight, serum parameters and adipose tissue weights.Results:The body weight of rats, as well as the serum levels of triglyceride and insulin, were all decreased after nicotine treatment; the weights of subcutaneous fat, visceral fat and periaortic fat were also decreased. The above indicators increased after withdrawal of nicotine.Conclusion:The established model can be used to study multiple pharmacological effects of nicotine; it can also be used for smoking and smoking cessation related studies.
Key words:  nicotine  smoking  tobacco use cessation  animal models  fats  bodymass  aorta