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膀胱癌组织中MMP-2、MMP 9、VEGF和RECK基因的表达及临床意义
鄢阳，郑军华*，许云飞，张海民，黄建华，彭波，高其若，黄国华，巢月根，孔宪国
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(同济大学附属第十人民医院泌尿外科，上海 200072)

摘要:

目的：观察MMP-2、MMP9、VEGF和RECK基因在膀胱癌组织中的表达，探讨膀胱癌转移可能的基因机制。方法：采用RTPCR法检测50例膀胱癌手术切除标本中MMP-2、MMP9、VEGF和RECK基因的表达，并分析其临床意义。结果：MMP-2、MMP9、VEGF和RECK在膀胱癌组织中的阳性表达率分别为80％、56%、78%和64%。膀胱癌组织MMP-2、MMP9和VEGF三种基因的表达水平高于癌周组织（P<0.05），而RECK基因的表达水平低于癌周组织（P<0.05）。膀胱癌组织中MMP-2、 MMP9和VEGF基因的表达水平与膀胱癌组织的病理分级分期密切相关，而RECK基因的表达水平与膀胱癌的分级分期无明显相关。结论：膀胱癌组织存在多基因的表达异常，其发生、发展及转移与多基因联合作用有关。

关键词: 膀胱肿瘤多基因表达肿瘤转移

DOI：10.3724/SP.J.1008.2007.01075

基金项目:国家自然科学基金（30270522），上海市科委基金（054119604）.

Expression of MMP-2, MMP 9, VEGF and RECK genes in bladder cancer and its clinical significance

YAN Yang, ZHENG Junhua*，XU Yunfei, ZHANG Haimin，HUANG Jianhua, PENG Bo, GAO Qiruo, HUANG Guohua, CHAO Yuegen，KONG Xianguo

(Department of Urology, The 10th People’s Hospital of Shanghai, Tongji University, Shanghai 200072,China)

Abstract:

Objective：To study the expression of MMP2, MMP9, vascular endothelial growth factor (VEGF) and RECK genes in bladder cancer tissues, so as to provide evidence for studying the molecular mechanism of bladder cancer metastasis. Methods: Reverse transcription polymerase chain reaction (RTPCR) was used to determine the expression of MMP2, MMP9, VEGF and RECK genes in 50 operative specimens of bladder cancer and their corresponding adjacent normal tissues; and the clinical significance of the expression was analyzed. Results: The positive rates of MMP2, MMP9, VEGF and RECK mRNA in the bladder cancer specimens were 80％, 56%, 78% and 64%, respectively. The expression levels of MMP2, MMP9 and VEGF mRNA in bladder cancer specimens were significantly higher than those in the paired adjacent normal bladder tissues（P<0.05）; the expression level of RECK genes in bladder cancer specimens was significantly lower than that in the normal tissue（P<0.05）. The expression levels of MMP2, MMP9 and VEGF was closely correlated with the clinical stages and grades of bladder cancer; there was no relation between the level of RECK expression and clinical stages and grades of the cancer. Conclusion: Abnormal expression of multigenes is present in bladder cancer tissues; the development, progression and metastasis of bladder cancer are associated with abnormal expression of multigenes.

Key words: bladder neoplasms expressions of multiple gene neoplasm metastasis