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催化信号放大法的改进
倪灿荣
0
(第二军医大学长海医院病理科,上海200433)
摘要:
目的:探讨酪胺与过氧化物酶发生酶促反应后,用热缓冲液洗以进一步提高和改善催化信号放大法(CSA)的敏感性和特异性。方法:应用7种不同类型的一抗,2张不同肿瘤的组织芯片,同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色,比较敏感性和特异性。结果:改进CSA法最为敏感,其次是标准CSA法、EnVision法和LsAB法,改进CSA法的敏感性较标准CSA法高2~3倍。4种方法背景染色基本一致,细胞定位准确。结论:改进后的CSA法的稳定性、敏感性好于标准CSA法。
关键词:  催化信号放大法 免疫组织化学 敏感性与特异性
DOI:10.3724/SP.J.1008.2007.00209
基金项目:
Modification of catalyzed signal amplification method
NI Can-rong
(Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
Abstract:
Objective:To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification (CSA). Methods: Using 7 different types of primary antibodies and 2 tumor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining and compared their sensitivities and specificities. Results: The modified CSA method was the most sensitive one among the 4 methods, followed by standard CSA, EnVision, and LsAB method. The sensitivity of modified CSA method was 2-3 times higher than that of standard CSA. The 4 methods had similar background staining and had exact localization of cells. Conclusion: The modified CSA is more stable and sensitive than traditional CSA staining method
Key words:  catalyzed signal amplification  immunohistochemistry  sensitivity and specificity