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C型利尿钠肽对AGEs抑制的利尿钠肽B受体及受体后信号的调节作用
曲卫邹俊杰石勇铨张春阳刘志民
0
(第二军医大学长征医院内分泌科,上海200003)
摘要:
目的:探讨C型利尿钠肽(CNP)对晚期糖基化终末产物(AGEs)抑制的利尿钠肽B受体(NPR—B)及受体后信号的影响。方法:体外培养人肾小球系膜细胞,分别用免疫荧光染色法、酶联免疫吸附分析法和流式细胞术观察CNP和AGEs作用下的NPR-B免疫荧光表达、细胞内cGMP水平和NF-κBp65活性亚基的表达。结果:CNP可以显著上调NPR—B的荧光表达、显著升高AGEs抑制的细胞内cGMP浓度(P〈0.001)及抑制AGEs刺激的NF-κBp65活性亚基的表达。结论:CNP能够调节AGEs影响的NPR—B/cGMP信号及NF-κB活性,并且可能通过NPR—B/cGMP途径发挥抑制NF-κB活性的作用。[著者文摘]
关键词:  利尿钠肽,C型 肾小球系膜细胞 糖基化终末产物
DOI:10.3724/SP.J.1008.2007.00758
基金项目:国家重点基础研究发展计划(973计划)(2005CB523304)
Influence of C-type natriuretic peptide on expression of natriuretic peptide B receptor suppressed by advanced glycation end products and on post-receptor signal regulation
QU Wei, ZOU Junjie, SHI Yong-quan, ZHANG Chun-yang, LIU Zhi-min
(Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China)
Abstract:
Objective:To investigate the influence of C-type natriuretic peptide(CNP) on the expression of natriuretic peptide B receptor(NPR B) and its cGMP signal induced by advanced glycation end products(AGEs) in human mesangial cells(HMC). Methods: HMCs were cultured in vitro. The expression of NPRB, intracellular concentration of cGMP and level of NF-κB p65 in HMC were measured by immunofluorescent staining, enzyme linked immunosorbem assay(ELISA) and flow cytometry, respectively. Results: CNP significantly upregulated the immunofluorescent intensity of NPR-B and the concentration of cGMP inhibited by AGEs(P〈0.001) ;CNP also inhibited the fluorescent intensity of NF-κB p65 stimulated by AGEs. Conclusion: CNP can regulate the expression of NPR-B/ cGMP and activity of NF-κB induced by AGEs through a NPR- B/cGMP dependent manner.[著者文摘]
Key words:  natriuretic peptide,C-type  mesangial cell  glycosylation end products