| 摘要: |
| 目的：观察不同磺脲类药物对糖尿病大鼠心肌组织磺脲类药物受体（SUR2）表达的影响.方法：选择自发性糖尿病GK大鼠作为2型糖尿病动物模型,随机分为6组（n=12）,即糖尿病对照组,胰岛素、格列本脲、格列吡嗪、格列齐特和格列美脲治疗组;另设正常对照组（n=12）.应用放射配体结合法检测SUR2,应用半定量RT-PCR方法检测SUR2 mRNA表达.结果：经不同磺脲类药物干预12周后,糖尿病组、胰岛素治疗组和正常对照组SUR2受体密度无差异,SUR2 mRNA表达水平较对照组稍下降,但无统计学差异;各磺脲类药物治疗组SUR2受体密度及mRNA表达水平较正常对照组和糖尿病组无明显变化.干预第24周,糖尿病大鼠SUR2 mRNA表达水平较第12周呈下降趋势,也低于同期正常对照大鼠,但仍无统计学差异;各磺脲类药物治疗组SUR2受体密度及 mRNA表达水平也变化不大.结论：在自发性糖尿病GK大鼠所表现出的糖尿病状态不影响心肌SUR2表达水平;治疗剂量的磺脲类药物对糖尿病大鼠心肌组织SUR2的表达无明显影响.[著者文摘] |
| 关键词: 糖尿病 心肌 磺脲类药物 |
| DOI：10.3724/SP.J.1008.2007.00761 |
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| 基金项目:上海市科委重大科技攻关基金（04DZ19507）. |
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| Effect of sulfonylurea compounds on expression of sulfonylurea receptor 2 in myocardium of diabetic Goto-Kakizaki rats |
| WU Guo-ting, XU Bei |
| (Department of Endocrinology, Shanghai Tenth People＇s Hospital, Tongji University, Shanghai 200072,China) |
| Abstract: |
| Objective： To observe effects of different sulfonylurea compounds on expression of sulfonylurea receptor 2 （SUR2）in myocardium of the Goto-Kakizaki（GK）rats. Methods： Spontaneous diabetic GK rat models were divided into 6 groups： the diabetes model group, the Glibenclamide group, the Glipizide group, the Gliclazide group, the Glimepiride group and the positive control group（treated with insulin）, with 12 rats in each group. A normal control group was also set up for comparison. The expression of SUR2 in myocardium of the GK rats was investigated by radioligand binding assay. SUR2 mRNA expression in the myocardial cells of rats was detected through reverse transcriptase-polymerase chain reaction （RT PCR）. Results： Twelve weeks later, no significant difference was found in the SUR2 receptor density（Bmax）and affinity（Kd） between the sulfonylurea treated groups and the other 3 groups （P〉0.05）. There was no significant difference in SUR2 mRNA expression between the diabetic, insulin-treated diabetic and control groups （P〉0.05）. Cardiac SUR2 mRNA levels were not significantly different between sulfonylureas treated diabetic and non-treated diabetic rats （P〉0.05）. Conclusion： The diabetes itself does not affect the sulfonylurea receptor（SUR2） expression in myocardial tissues. Sulfonylureas at treatment dosage have no effect on receptor expression of SUR2.[著者文摘] |
| Key words: diabetes mellitus myocardium sulfonylureas |
