| 摘要: |
| 目的：观察不同时限失神经状态下人环杓后肌成肌调节因子myogenin的表达变化，为喉神经修复时限的选择奠定基础。方法：取38例喉返神经完全离断患者的环杓后肌标本，按神经离断时限分为4组：失神经6~12个月（n=12）、1~2年（n=10）、2~3年（n=8）及>3年组（n=8），另取12例正常环杓后肌标本作为对照，各组患者的年龄、性别比例具有可比性。免疫荧光染色、实时定量PCR法比较各组环杓后肌myogenin蛋白及mRNA的表达。结果：免疫荧光染色结果显示：myogenin阳性表达主要定位于失神经3年内的环杓后肌肌细胞核，对照组正常环杓后肌基本无阳性表达；与正常对照组相比，失神经6~12个月组肌细胞核myogenin表达程度及阳性细胞百分比升高，1~2年组表达最高，2~3年组逐渐下降，但仍显著高于对照组（P均<0.01），而3年以上组基本无阳性表达，与对照组无显著差异。实时定量PCR结果表明：对照组myogenin mRNA未表达，失神经1~2年组myogenin mRNA表达水平为6~12个月组的4倍，失神经2~3年组为其64倍，失神经3年以上组仅为其1/2（P均<0.01）。结论：失喉返神经支配的环杓后肌肌纤维3年内有较大的再生潜力。 |
| 关键词: 环杓后肌 喉返神经 创伤和损伤 myogenin |
| DOI：10.3724/SP.J.1008.2007.00951 |
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| 基金项目:国家自然科学基金（30672295）. |
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| Changes of myogenin expression in long-term denervated human posterior cricoarytenoid muscles |
| ZHANG Xian1,ZHENG Hong-liang2, CHEN Shi-cai2 |
| (1. Department of Otorhinolaryngology,Fuzhou General Hospital,PLA Nanjing Military Area Command,Fuzhou 350025, China; 2.Department of Otorhinolaryngology, Changhai Hospital, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective：To investigate the change in myogenin expression at different time in long-term denervated human posterior cricoarytenoid muscles（PCAMs）, so as to provide a theoretical basis for timing of reinnervation. Methods: Thirty-eight specimens of denervated human PCAMs were divided into 4 groups according to the period of denervation: 6-12 months denervation group（n=12） , 1-2 years denervation group（n=10）, 2-3 years denervation group（n=8）, and over 3 years denervation group（n=8）. Another 12 specimens of normal PCAMs served as control. The patients in all groups were age- and sex-matched. The expression of myogenin protein and mRNA was studied using immunofluorescence staining and real-time PCR analysis, respectively. Results: Immunofluorescence staining showed that the positive myogenin expression was mainly found in the myonuclei of PCAMs with a denervation period less than 3 years; no positive staining was found in the myonuclei of control group. The expression of myogenin in myonuclei and the ratio of positive cells were up-regulated in the 6-12 month denervation group compared with those in the control group; the expression and the ratio peaked in 1-2 years denervation group and decreased again in the 2-3 years denervation group, but was still significantly higher than those of the control group (all P<0.01). There was hardly any expression of myogenin 3 years after denervation. Results of RT-PCR showed no myogenin mRNA expression in the control group; the expression in 1-2 years, 2-3 years, and more than 3 years denervation groups were 4 times, 64 times, and half that of the 6-12 months denervation group, respectively (all P<0.05). Conclusion: It is indicated that there is a potential for muscle regeneration within 3 years of denervation. |
| Key words: posterior cricoarytenoid muscles recurrent laryngeal nerve wounds and injuries myogenin |
