| 摘要: |
| 目的:观察过氧化物酶体增殖物激活受体γ(PPARγ)在不同年龄大鼠肾组织中的表达与分布,并进一步探讨其与衰老过程中氧化应激的关系。方法:选用3个月龄、12个月龄、24个月龄自然衰老SD大鼠为模型, Western印迹分析PPARγ在不同年龄大鼠肾组织中的表达,通过测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性反映肾组织细胞抗氧化能力。结果: 应用Western印迹检测肾脏PPARγ的蛋白表达,发现PPARγ蛋白在大鼠肾组织中表达随月龄下降,3月龄肾组织中PPARγ表达明显高于24个月龄大鼠(P<0.01);SOD、GSH-PX活性均随月龄增加明显下降(P<0.01),两者的活性均与PPARγ蛋白的表达呈明显正相关(r=0.900,r=0.890,P均<0.01)。结论:PPARγ在大鼠肾组织衰老过程中呈下降趋势,这种变化与肾脏组织抗氧化应激能力下降有关。 |
| 关键词: 肾 过氧化物酶增殖物激活受体-γ ；衰老； 氧化应激 |
| DOI：10.3724/SP.J.1008.2008.01293 |
| 投稿时间：2008-01-08修订日期：2008-10-05 |
| 基金项目:国家重点基础研究发展计划(“973”计划)(2007CB07400). |
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| Expression of peroxisome proliferators-activate receptor γ in renal tissue of aging rats and its relationship with oxidative stress |
| ZHANG Ying-wei,SHI Duo,XIONG Xi-shan,ZHANG Hong,HU Hui-min,MEI Chang-lin* |
| (Department of Nephrology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China) |
| Abstract: |
| Objective:To investigate the expression of peroxisome proliferator-activate receptor γ(PPARγ) in the kidneys of rats at different ages,so as to study the possible association of PPARγ expression with oxidative stress in the aging process.Methods: Rat aging model was established using 3-,12-,and 24-month old rats.Western blotting was used to determining the protein expression of PPARγ.The changes anti-oxidative ability of renal tissue were studied by determining the activities of superoxidedismutase(SOD)and glutatio-neperoxidase(GSH-PX).Results: Western blotting assay showed that the renal expression of PPARγ protein decreased with the aging of rats,with that of 3-month group significantly higher than that of the 24-month group(P<0.01).The activities of SOD and GSH-PX deceased with the aging of rats(P<0.01).Both the SOD activities were positively correlated with the expression of PPARγ protein(r=0.90,r=0.89,P<0.01) Conclusion: Renal PPARγ has a decreasing tendency during the aging process of rats,which is correlated with the down-regulation of anti-oxidative ability of renal tissue. |
| Key words: kidney peroxisome proliferator-activated receptor γ aging oxidative stress |
