| 摘要: |
| 目的：观察苯甲酸雌二醇对γ射线诱导小鼠骨髓造血细胞凋亡的影响, 以探讨其抗放射作用机制。方法：昆明种小鼠, 随机分为正常组、单纯照射组和用药照射组3组，每组各15只。单纯照射组小鼠用γ射线照射4.0 Gy；用药照射组于照射前10 d每只小鼠肌内注射0.1 mg 苯甲酸雌二醇，同时用γ射线照射4.0 Gy；正常对照组不作任何处理。采用DNA琼脂糖凝胶电泳检测骨髓造血细胞DNA裂解片段，采用流式细胞术观察各组小鼠照射后4 h、8 h、12 h骨髓造血细胞凋亡、Fas 和Bcl-2表达的变化情况。结果：γ射线照后8 h，骨髓造血细胞DNA裂解片段增加，出现了典型DNA“梯状”条带，而用药照射组和正常对照组均未见明显“梯状”条带。照射后4 h、8 h、12 h，用药照射组骨髓造血细胞凋亡率均明显低于单纯照射组(P<0.01)；Fas表达较正常对照组略有增加,并未出现单纯照射组所呈现典型的高峰期，与单纯照射组比较显著降低(P<0.01)；Bcl-2表达不降反而升高,与单纯照射组相比差异显著(P<0.01)。结论：苯甲酸雌二醇可能是通过下调Fas和上调Bcl-2的表达抑制了γ射线诱导的小鼠骨髓造血细胞凋亡。 |
| 关键词: 雌二醇 造血细胞 细胞凋亡 辐射损伤 Fas Bcl-2 |
| DOI：10.3724/SP.J.1008.2008.00648 |
| 投稿时间：2008-01-14修订日期：2008-05-21 |
| 基金项目: |
|
| Effects of estradiol on radiation-induced apoptosis of bone marrow hematopoietic cells in mice |
| LI Bai-long,CAI Jian-ming*,CUI Jian-guo,XIANG Ying-song,GAO Fu,YANG Ru-jun，HUANG Yue-cheng |
| (Department of Radiation Medicine,Faculty of Naval Medicine,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective：To investigate the effects of estradiol on 60Co γ-ray induced apoptosis of bone marrow hematopoietic cells of mice,and to discuss the related anti-irradiation mechanism.Methods: KM mice were randomly divided into 3 groups (15 mice/each group): control group (without radiation),pure radiation group and estradiol + radiation group (ER group).The pure radiation group was irradiated by 4.0 Gy γ-ray at a dose rate of 1.15Gy/min; the ER group was administered with 0.1 mg estradiol (IM) at 10 days before 4.0 Gy γ-ray radiation; and the control group received no special treatment.The apoptotic DNA segments of bone marrow hematopoietic cells were analyzed by DNA agarose gel electrophoresis; flow cytometry was used to examine the apoptosis rate of cells and expression of Fas and Bcl-2 at 4 h,8 h,and 12 h after irradiation.Results: Eight hours after radiation,the apoptotic DNA segments were obviously increased and apoptotic DNA ladder appeared,which was not seen in the other 2 groups.The apoptosis rate of bone marrow hematopoietic cells in ER group was significantly lower than that in the pure radiation group at 4,8, and 12 h after irradiation(P<0.01).Fas expression was only slightly elevated in the ER group than in the control group, but was markedly lower than that in pure radiation group(P<0.01),and showed no typical crest-time； Bcl-2 expression was significantly higher in the ER group than in the pure radiation group(P<0.01).Conclusion: Estradiol can decrease the apoptosis of mice bone marrow hematopoietic cells induced by γ-ray,probably through down-regulation of Fas and up-regulation of Bcl-2 expression. |
| Key words: extradiol hematopoietic cell apoptosis radiation injuries Fas Bcl-2 |
