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雷公藤多苷对结肠炎小鼠结肠组织IL-23、IL-17和IL-12表达的影响
肖南平1,欧阳钦1*,谭柯2
0
(1.四川大学华西医院消化内科,成都 610041*2.四川大学华西公共卫生学院卫生统计学教研室,成都 610041)
摘要:
目的:观察雷公藤多苷( GTT)、美沙拉嗪对三硝基苯磺酸(TNBS)诱导的急性结肠炎小鼠结肠组织中IL-23、IL-17、IL-12表达的影响,探讨其可能的作用机制。方法:C57BL/6小鼠随机分为4组:正常对照组、模型组、美沙拉嗪组、雷公藤多苷组,后3组应用TNBS建立小鼠急性结肠炎模型,模型组不作其他处理,雷公藤多苷组和美沙拉嗪组于造模前4 d开始每天给予雷公藤多苷灌胃或美沙拉嗪灌肠液灌肠直到实验结束,正常对照组不作任何特殊处理。各组小鼠均于TNBS灌肠后48 h处死,检测各组小鼠结肠组织大体、组织学损伤评分及髓过氧化物酶(MPO)活性;ELISA法检测结肠组织IL-23 p19、IL-17蛋白含量,实时荧光定量RT-PCR检测结肠组织IL-23 p19、IL-17、IL-12 p35的mRNA表达。结果:美沙拉嗪组和雷公藤多苷组小鼠结肠大体及组织学损伤记分、MPO活性明显低于模型组(P<0.05);模型组结肠组织IL-23 p19、IL-17、IL-12 p35 mRNA表达水平明显高于正常对照组、美沙拉嗪组、雷公藤多苷组(P<0.05);模型组结肠组织IL-23 p19、IL-17蛋白水平明显高于正常对照组、美沙拉嗪组、雷公藤多苷组(P<0.05),后三者间无统计学差异。结论:雷公藤多苷可能通过非选择性抑制IL-23 p19、IL-17、IL-12 p35的表达来抑制结肠炎小鼠的炎症反应,效果与美沙拉嗪类似。
关键词:  结肠炎  IL-23  IL-17  IL-12  雷公藤多苷  美沙拉嗪  三硝基苯磺酸
DOI:10.3724/SP.J.1008.2008.01069
投稿时间:2008-01-21修订日期:2008-03-20
基金项目:
Influence of glucosidorum tripterygii tororum on expression of interleukin-23, interleukin-17 and interleukin-12 in colonic tissues of mice with colitis
XIAO Nan-ping1,OUYANG Qin1*,TAN Ke2
(1. Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu 610041, China*2. Department of Health Statistics, West China School of Public Health, Sichuan University, Chengdu 610041)
Abstract:
Objective:To study the influence of glucosidorum tripterygii tororum (GTT) and Mesalazine on the expression of IL-23, IL-17 and IL-12 in the colonic tissues of mice with rinitrobenzene sulphonic acid (TNBS)-induced acute colitis, and to study the possible mechanism. Methods: The C57BL/6 mice were divided into 4 groups, namely, a control group, a model group, a Mesalazine group and a GTT group. Colitis was induced by TNBS in the last 3 groups. The mice in the model group received no additional treatment; those in the GTT group received GTT daily by oral gavage 4 days before exposure to TNBS till the end of the experiment, and those in the Mesalazine group received Mesalazine enema solution daily 4 days before exposure till the end of the experiment. All the mice were sacrificed at 48 h after enema with TNBS. The macroscopic and histological scores of colon damage and myeloperoxidase (MPO) activity in colonic tissue were evaluated in every group. IL-23p19 and IL-17 contents in colonic tissues were measured by ELISA; the expression of IL-23p19, IL-17 and IL-12p35 mRNA in colonic tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-FQ-PCR) with SYBR Green Ⅰ.Results: Compared with the model group, GTT group and Mesalazine group had significantly lower macroscopic and histological scores and MPO activity (P<0.05). Expression of IL-23p19, IL-17 and IL-12p35 mRNA in the colonic tissues of the model group was significantly higher than that of the other 3 groups(P<0.05); the expression of IL-23p19, IL-17 protein was significant higher in the model group than that in the other 3 groups (P<0.05), with no significant difference found between the later 3 groups.Conclusion: GTT, like Mesalazine, can effectively inhibit inflammation in mice with TNBS-induced
Key words:  colitis  interleukin-23  interleukin-17  interleukin-12  glucosidorum tripterygii tororum  mesalazine  trinitrobenzenesulfonic acid