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| 斑马鱼rnf141基因结构与表达分析 |
| 李亚1,王伦安2*,王玉明1,杨平1,潘克俭1,黄明孔3 |
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| (1.成都医学院生物化学与分子生物学教研室,成都 610081;2.成都医学院科研中心,成都 610081;3.四川生殖卫生学院附属医院泌尿科,成都 610041) |
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| 摘要: |
| 目的:探讨斑马鱼rnf141基因在物种间进化以及在斑马鱼胚胎发育和成体中表达分布特点。方法:采用生物信息学方法分析斑马鱼rnf141基因结构特征;运用胚胎整体原位杂交和多组织RT-PCR技术分别检测斑马鱼胚胎发育不同阶段和成体各组织rnf141基因表达情况。结果:斑马鱼RNF141蛋白与人类和小鼠同源蛋白(ZNF230、Znf230)分别具有79.8%、78.5%的同源性,其中C3HC4型锌指结构域及其保守的半胱氨酸和组氨酸残基组成与人类(或小鼠)的同源性分别为84.8%、100%;而且,斑马鱼RNF141蛋白与人类ZNF230蛋白在C3HC4型锌指区域具有完全相同的二级结构。杂交结果显示,rnf141基因在斑马鱼胚胎中广泛表达,尤以端脑、小脑和后脑表达丰度为高。RT-PCR结果表明,rnf141基因在受检的成体组织中均有表达,但在睾丸组织中表达丰度较高。结论:斑马鱼rnf141基因为脊椎动物保守基因,并可能在胚胎发育和精子发生过程中起着重要作用。 |
| 关键词: 斑马鱼 rnf141基因 整体原位杂交 |
| DOI：10.3724/SP.J.1008.2008.00967 |
| 投稿时间：2008-01-28修订日期：2008-05-25 |
| 基金项目: |
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| Expression and molecular characterization of zebrafish rnf141 gene |
| LI Ya1,WANG Lun-an2*,WANG Yu-ming1,YANG Ping1,PAN Ke-jian1,HUANG Ming-kong3 |
| (1.Department of Biochemistry and Molecular Biology,Chengdu Medical College,Chengdu 610081;2.Center of Scientific Research,Chengdu Medical College,Chengdu 610081;3.Department of Urology,Affiliated Hospital of Reproduction Health College of Sichuan Province,Chengdu 610041) |
| Abstract: |
| Objective:To explore the molecular evolution of zebrafish rnf141 gene and its expression in the embryo and adult zebrafish.Methods: The molecular characteristics of zebrafish rnf141 gene were analyzed through a bioinformatics approach.Expression of rnf141 gene in the embryos of different developmental stages and tissues of adult fish were examined by whole-mount in situ hybridization and multiple-tissue RT-PCR technique.Results: We noticed that zebrafish RNF141 protein shared a 79.8% and 78.5% homologies to its homologues in human and mouse (ZNF230,Znf230),respectively,especially that the C3HC4-type domain and the same conserved cysteine and histidine residues shared a 84.8% and 100% homologies to its homologues in human or mouse.Moreover,the secondary structure of C3HC4-type domain in zebrafish RNF141 protein was the same as that in its human homologue.Whole-mount in situ hybridization showed that zebrafish rnf141 gene expressed ubiquitously during embryogenesis,with higher expression found in the telencephalon,cerebellum and hindbrain.RT-PCR revealed rnf141 gene expression in all the adult tissues detected,and abundance was observed in the testicular tissue.Conclusion: These data suggest that zebrafish rnf141 gene is conservative in the vertebrate and might play important roles in embryogenesis and spermatogenesis. |
| Key words: zebrafish rnf141 whole-mount in situ hybridization |
